Identification of herpesvirus transcripts from genomic regions around the replication origins

Long-read sequencing (LRS) techniques enable the identification of full-length RNA molecules in a single run eliminating the need for additional assembly steps. LRS research has exposed unanticipated transcriptomic complexity in various organisms, including viruses. Herpesviruses are known to produc...

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Bibliographic Details
Main Authors: Torma Gábor
Tombácz Dóra
Csabai Zsolt
Almsarrhad Islam
Nagy Gergely Ármin
Kakuk Balázs
Gulyás Gábor
Spires Lauren McKenzie
Gupta Ishaan
Fülöp Ádám
Dörmő Ákos
Prazsák István
Mizik Máté Levente
Dani Virág Éva
Csányi Viktor
Harangozó Ákos
Zádori Zoltán
Toth Zsolt
Boldogkői Zsolt
Format: Article
Published: 2023
Series:SCIENTIFIC REPORTS 13 No. 1
Subjects:
doi:10.1038/s41598-023-43344-y

mtmt:34171230
Online Access:http://publicatio.bibl.u-szeged.hu/29085
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Summary:Long-read sequencing (LRS) techniques enable the identification of full-length RNA molecules in a single run eliminating the need for additional assembly steps. LRS research has exposed unanticipated transcriptomic complexity in various organisms, including viruses. Herpesviruses are known to produce a range of transcripts, either close to or overlapping replication origins (Oris) and neighboring genes related to transcription or replication, which possess confirmed or potential regulatory roles. In our research, we employed both new and previously published LRS and short-read sequencing datasets to uncover additional Ori-proximal transcripts in nine herpesviruses from all three subfamilies (alpha, beta and gamma). We discovered novel long non-coding RNAs, as well as splice and length isoforms of mRNAs. Moreover, our analysis uncovered an intricate network of transcriptional overlaps within the examined genomic regions. We demonstrated that herpesviruses display distinct patterns of transcriptional overlaps in the vicinity of or at the Oris. Our findings suggest the existence of a ‘super regulatory center’ in the genome of alphaherpesviruses that governs the initiation of both DNA replication and global transcription through multilayered interactions among the molecular machineries.
Physical Description:25
ISSN:2045-2322