Dependence of Chlorophyll Fluorescence Quenching on the Lipid-to-Protein Ratio in Reconstituted Light-Harvesting Complex II Membranes Containing Lipid Labels
The quenching of chlorophyll-a fluorescence was investigated in plant light-harvesting complex II (LHCII) embedded in reconstituted membranes containing thylakoid lipids and a lipid label. The proteoliposomes were further separated by density and the protein and lipid contents of the fractions were...
Elmentve itt :
| Szerzők: | |
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2019
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| Sorozat: | CHEMICAL PHYSICS
522 |
| Tárgyszavak: | |
| doi: | 10.1016/j.chemphys.2019.03.012 |
| mtmt: | 30629753 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/27330 |
| Tartalmi kivonat: | The quenching of chlorophyll-a fluorescence was investigated in plant light-harvesting complex II (LHCII) embedded in reconstituted membranes containing thylakoid lipids and a lipid label. The proteoliposomes were further separated by density and the protein and lipid contents of the fractions were quantified spectrophotometrically, allowing tighter control over the L/P ratios in a wide range of values. Using time-resolved fluorescence, we found a strong correlation between the fluorescence quenching and the L/P ratio, in line with other studies reporting progressive quenching at low L/P ratios, presumably triggered by self-clustering in the membrane. The average fluorescence lifetimes decreased to 0.3 ns at L/P ratios below 50:1; these values are comparable to the quenching observed in plants under excess-light conditions and are accompanied by a similar far-red fluorescence signature. It is hypothesized that plants can exploit the intrinsic quenching propensity of LHCII-only membrane domains to safely store extra antenna units. |
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| Terjedelem/Fizikai jellemzők: | 242-248 |
| ISSN: | 0301-0104 |