The Inhibition of the Small-Conductance Ca2+-Activated Potassium Channels Decreases the Sinus Node Pacemaking during Beta-Adrenergic Activation
Sinus pacemaking is based on tight cooperation of intracellular Ca2+ handling and surface membrane ion channels. An important player of this synergistic crosstalk could be the small-conductance Ca2+-activated K+-channel (ISK) that could contribute to the sinoatrial node (SAN) pacemaking driven by th...
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2022
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| Sorozat: | PHARMACEUTICALS
15 No. 3 |
| Tárgyszavak: | |
| doi: | 10.3390/ph15030313 |
| mtmt: | 32743636 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/23922 |
| LEADER | 02338nab a2200289 i 4500 | ||
|---|---|---|---|
| 001 | publ23922 | ||
| 005 | 20220401102715.0 | ||
| 008 | 220401s2022 hu o 0|| Angol d | ||
| 022 | |a 1424-8247 | ||
| 024 | 7 | |a 10.3390/ph15030313 |2 doi | |
| 024 | 7 | |a 32743636 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
| 041 | |a Angol | ||
| 100 | 1 | |a Bitay Gergő | |
| 245 | 1 | 4 | |a The Inhibition of the Small-Conductance Ca2+-Activated Potassium Channels Decreases the Sinus Node Pacemaking during Beta-Adrenergic Activation |h [elektronikus dokumentum] / |c Bitay Gergő |
| 260 | |c 2022 | ||
| 300 | |a Terjedelem: 15 p.-Azonosító: 313 | ||
| 490 | 0 | |a PHARMACEUTICALS |v 15 No. 3 | |
| 520 | 3 | |a Sinus pacemaking is based on tight cooperation of intracellular Ca2+ handling and surface membrane ion channels. An important player of this synergistic crosstalk could be the small-conductance Ca2+-activated K+-channel (ISK) that could contribute to the sinoatrial node (SAN) pacemaking driven by the intracellular Ca2+ changes under normal conditions and beta-adrenergic activation, however, the exact role is not fully clarified. SK2 channel expression was verified by immunoblot technique in rabbit SAN cells. Ionic currents and action potentials were measured by patch-clamp technique. The ECG R-R intervals were obtained by Langendorff-perfusion method on a rabbit heart. Apamin, a selective inhibitor of SK channels, was used during the experiments. Patch-clamp experiments revealed an apamin-sensitive current. When 100 nM apamin was applied, we found no change in the action potential nor in the ECG R-R interval. In experiments where isoproterenol was employed, apamin increased the cycle length of the SAN action potentials and enhanced the ECG R-R interval. Apamin did not amplify the cycle length variability or ECG R-R interval variability. Our data indicate that ISK has no role under normal condition, however, it moderately contributes to the SAN automaticity under beta-adrenergic activation. | |
| 650 | 4 | |a Farmakológia és gyógyszerészet | |
| 700 | 0 | 1 | |a Tóth Noémi |e aut |
| 700 | 0 | 1 | |a Déri Szilvia |e aut |
| 700 | 0 | 1 | |a Szlovák Jozefina |e aut |
| 700 | 0 | 1 | |a Kohajda Zsófia |e aut |
| 700 | 0 | 1 | |a Varró András |e aut |
| 700 | 0 | 1 | |a Nagy Norbert |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/23922/1/BitayG2022.pdf |z Dokumentum-elérés |