OCT1 - a yeast mitochondrial thiolase involved in the 3-oxoadipate pathway

The 3-oxoacyl-CoA thiolases catalyze the last step of the fatty acid β-oxidation pathway. In yeasts and plants, this pathway takes place exclusively in peroxisomes, whereas in animals it occurs in both peroxisomes and mitochondria. In contrast to baker's yeast Saccharomyces cerevisiae, yeast sp...

Teljes leírás

Elmentve itt :
Bibliográfiai részletek
Szerzők: Vrzoňová Romana
Tóth Renáta
Siváková Barbara
Moťovská Anna
Gaplovská-Kyselá Katarína
Baráth Peter
Tomáška Ľubomír
Gácser Attila
Gabaldón Toni
Nosek Jozef
Neboháčová Martina
Dokumentumtípus: Cikk
Megjelent: 2021
Sorozat:FEMS YEAST RESEARCH 21 No. 5
doi:10.1093/femsyr/foab034

mtmt:32119106
Online Access:http://publicatio.bibl.u-szeged.hu/22019
Leíró adatok
Tartalmi kivonat:The 3-oxoacyl-CoA thiolases catalyze the last step of the fatty acid β-oxidation pathway. In yeasts and plants, this pathway takes place exclusively in peroxisomes, whereas in animals it occurs in both peroxisomes and mitochondria. In contrast to baker's yeast Saccharomyces cerevisiae, yeast species from the Debaryomycetaceae family also encode a thiolase with predicted mitochondrial localization. These yeasts are able to utilize a range of hydroxyaromatic compounds via the 3-oxoadipate pathway the last step of which is catalyzed by 3-oxoadipyl-CoA thiolase and presumably occurs in mitochondria. In this work, we studied Oct1p, an ortholog of this enzyme from Candida parapsilosis. We found that the cells grown on a 3-oxoadipate pathway substrate exhibit increased levels of the OCT1 mRNA. Deletion of both OCT1 alleles impairs the growth of C. parapsilosis cells on 3-oxoadipate pathway substrates and this defect can be rescued by expression of the OCT1 gene from a plasmid vector. Subcellular localization experiments and LC-MS/MS analysis of enriched organellar fraction-proteins confirmed the presence of Oct1p in mitochondria. Phylogenetic profiling of Oct1p revealed an intricate evolutionary pattern indicating multiple horizontal gene transfers among different fungal groups.
Terjedelem/Fizikai jellemzők:Terjedelem: 12-Azonosító: foab034
ISSN:1567-1356