Hypoxia and inflammation in the release of VEGF and interleukins from human retinal pigment epithelial cells
PURPOSE: Retinal diseases are closely associated with both decreased oxygenation and increased inflammation. It is not known if hypoxia-induced vascular endothelial growth factor (VEGF) expression in the retina itself evokes inflammation, or whether inflammation is a prerequisite for the development...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2017
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| Sorozat: | GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
255 No. 9 |
| doi: | 10.1007/s00417-017-3711-0 |
| mtmt: | 3255500 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/20340 |
| LEADER | 02777nab a2200277 i 4500 | ||
|---|---|---|---|
| 001 | publ20340 | ||
| 005 | 20210125123620.0 | ||
| 008 | 210125s2017 hu o 0|| zxx d | ||
| 022 | |a 0721-832X | ||
| 024 | 7 | |a 10.1007/s00417-017-3711-0 |2 doi | |
| 024 | 7 | |a 3255500 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
| 041 | |a zxx | ||
| 100 | 1 | |a Arjamaa Olli | |
| 245 | 1 | 0 | |a Hypoxia and inflammation in the release of VEGF and interleukins from human retinal pigment epithelial cells |h [elektronikus dokumentum] / |c Arjamaa Olli |
| 260 | |c 2017 | ||
| 300 | |a 1757-1762 | ||
| 490 | 0 | |a GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY |v 255 No. 9 | |
| 520 | 3 | |a PURPOSE: Retinal diseases are closely associated with both decreased oxygenation and increased inflammation. It is not known if hypoxia-induced vascular endothelial growth factor (VEGF) expression in the retina itself evokes inflammation, or whether inflammation is a prerequisite for the development of neovascularization. METHODS: Human ARPE-19 cell line and primary human retinal pigment epithelium (RPE) cells were used. ARPE-19 cells were kept either under normoxic (24 h or 48 h) or hypoxic conditions (1% O2, 24 h). Part of the cells were re-oxygenated (24 h). Some ARPE-19 cells were additionally pre-treated with bacterial lipopolysaccharide (LPS). The levels of IL-6, IL-8, IL-1beta, and IL-18 were determined from medium samples by an enzyme-linked immunosorbent assay (ELISA) method. Primary human RPE cells were exposed to hypoxia for 24 h, and the subsequent release of IL-6 and IL-8 was measured with ELISA. VEGF secretion from ARPE-19 cells was determined up to 24 h. RESULTS: Hypoxia induced significant (P < 0.01) increases in the levels of both IL-6 and IL-8 in ARPE-19 cells, and LPS pre-treatment further enhanced these responses. Hypoxia exposure did not affect the IL-1beta or IL-18 release irrespective of LPS pre-treatment. If primary RPE cells were incubated for 4 h in hypoxic conditions, IL-6 and IL-8 concentrations were increased by 7 and 8-fold respectively. Hypoxia increased the VEGF secretion from ARPE-19 cells in a similar manner with or without pre-treatment with LPS. CONCLUSIONS: Hypoxia causes an inflammatory reaction in RPE cells that is potentiated by pre-treatment with the Toll-like receptor-activating agent, LPS. The secretion of VEGF from these cells is regulated directly by hypoxia and is not mediated by inflammation. | |
| 700 | 0 | 1 | |a Aaltonen Vesa |e aut |
| 700 | 0 | 1 | |a Piippo Niina |e aut |
| 700 | 0 | 1 | |a Csont Tamás Bálint |e aut |
| 700 | 0 | 1 | |a Petrovski Goran |e aut |
| 700 | 0 | 1 | |a Kaarniranta Kai |e aut |
| 700 | 0 | 1 | |a Kauppinen Anu |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/20340/1/Arjamaa2017_Article_HypoxiaAndInflammationInTheRel.pdf |z Dokumentum-elérés |