Dynamic on-column eluent modification A novel strategy for peak resolution enhancement. Application to the preparative separation of ecdysteroid isomers /
A novel LC method was applied to enhance the peak resolution of two ecdysteroids: 20-hydroxyecdysone and its 3-epimer. An isocratic solvent system of methanol-water (6:4, v/v) was used on a C18 column, and 100 mu L of water was injected during the development in such a manner that the eluted solvent...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2008
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| Sorozat: | CHROMATOGRAPHIA
67 No. 9-10 |
| doi: | 10.1365/s10337-008-0557-y |
| mtmt: | 1723535 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/20049 |
| Tartalmi kivonat: | A novel LC method was applied to enhance the peak resolution of two ecdysteroids: 20-hydroxyecdysone and its 3-epimer. An isocratic solvent system of methanol-water (6:4, v/v) was used on a C18 column, and 100 mu L of water was injected during the development in such a manner that the eluted solvent peak appeared exactly between the two overlapping peaks. This resulted in the increased retardation of the later-eluted peak, and in a good separation of the two compounds within 4.5 min. |
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| Terjedelem/Fizikai jellemzők: | 767-772 |
| ISSN: | 0009-5893 |