Grapefruit Seed Extract Inhibits the Formation of Amyloid-like Fibrils by Trypsin in Aqueous Ethanol

Several natural compounds deriving from plants are known to be efficient anti-amyloid aggregation agents. In this study, anti-aggregation activity of grapefruit seed extract was investigated using trypsin as a model protein in aqueous ethanol at pH 7.0. Using turbidity measurement, Congo red (CR) bi...

Teljes leírás

Elmentve itt :
Bibliográfiai részletek
Szerzők: Kasi Phanindra Babu
Borics Attila
Varga Mónika
Endre Gábor
Molnár Kinga
László Lajos
Kotormán Márta
Dokumentumtípus: Cikk
Megjelent: 2018
Sorozat:NATURAL PRODUCT COMMUNICATIONS 13 No. 11
mtmt:30418445
Online Access:http://publicatio.bibl.u-szeged.hu/19377
Leíró adatok
Tartalmi kivonat:Several natural compounds deriving from plants are known to be efficient anti-amyloid aggregation agents. In this study, anti-aggregation activity of grapefruit seed extract was investigated using trypsin as a model protein in aqueous ethanol at pH 7.0. Using turbidity measurement, Congo red (CR) binding assay, electronic circular dichroism (ECD) and transmission electron microscopy (TEM), we found that grapefruit seed extract has ability to inhibit trypsin amyloidlike fibril formation in vitro, and effectiveness increases with growing concentration of grapefruit seed extract. The total phenolic content of it was determined. The results showed that in addition to the polyphenolic compounds some other compounds are also responsible for the fibril formation inhibitory effect. We indicated it for the first time that limonin has anti-fibrillation effect.
Terjedelem/Fizikai jellemzők:1437-1440
ISSN:1934-578X