Kupffer cell phagocytosis blockade decreases morbidity in endotoxemic rats with obstructive jaundice

Objective and design: The consequences of Kupffer cell phagocytosis blockade were studied in endotoxemic rats with obstructive jaundice. Material or Subjects: 159 male Wistar rats. Treatment: Obstructive jaundice was induced by bile duct ligation (BDL). Gadolinium chloride (1 mg/100g iv) was given 6...

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Main Authors: Lázár György ifj
Paszt Attila
Kaszaki József
Duda Ernő
Szakács Júlia
Tiszlavicz László
Boros Mihály
Balogh Ádám
Lázár György
Format: Article
Published: 2002
Series:INFLAMMATION RESEARCH 51 No. 10
doi:10.1007/PL00012421

mtmt:1320830
Online Access:http://publicatio.bibl.u-szeged.hu/18765
Description
Summary:Objective and design: The consequences of Kupffer cell phagocytosis blockade were studied in endotoxemic rats with obstructive jaundice. Material or Subjects: 159 male Wistar rats. Treatment: Obstructive jaundice was induced by bile duct ligation (BDL). Gadolinium chloride (1 mg/100g iv) was given 6 days after BDL to inhibit Kupffer cell activity and the animals were challenged with 1 mug/g endotoxin 24 h later. Methods: Endotoxin sensitivity, tumor necrosis factor-a and interleukin-6 production were studied, liver and lung injury were assessed by neutrophil infiltration assay, tissue adenosine triphosphate, aspartate aminotransferase, alanine aminotransferase level determinations and histology, respectively. For statistics non-parametric methods were used. Results: BDL sensitized the animals to endotoxin, increased endotoxin-induced tumor necrosis factor-a and interleukin-6 production and reduced ATP contents of the liver and the lung. Kupffer cell blockade significantly increased the resistance against endotoxin, diminished the inflammatory cytokine release and reduced endotoxin-induced tissue injury in BDL animals. Conclusion: Attenuation of Kupffer cell function decreases endotoxin-induced lethality and morbidity in obstructive jaundice.
Physical Description:511-518
ISSN:1023-3830