A Novel Method for in vivo Visualization of the Microcirculation of the Mandibular Periosteum in Rats
OBJECTIVE: The periosteum plays an important role in bone physiology, but observation of its microcirculation is greatly limited by methodological constraints at certain anatomical locations. The present study was conducted to develop a microsurgical procedure which provides access to the mandibular...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2014
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| Sorozat: | MICROCIRCULATION
21 No. 6 |
| doi: | 10.1111/micc.12128 |
| mtmt: | 2701164 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/18393 |
| Tartalmi kivonat: | OBJECTIVE: The periosteum plays an important role in bone physiology, but observation of its microcirculation is greatly limited by methodological constraints at certain anatomical locations. The present study was conducted to develop a microsurgical procedure which provides access to the mandibular periosteum in rats. METHODS: Comparisons of the microcirculatory characteristics with those of the tibial periosteum were performed to confirm the functional integrity of the microvasculature. The mandibular periosteum was reached between the facial muscles and the anterior surface of the superficial masseter muscle at the external surface of the mandibular corpus; the tibial periosteum was prepared by dissecting the covering muscles at the anteromedial surface. Intravital fluorescence microscopy was used to assess the leukocyte-endothelial interactions and the red blood cell velocity in the tibial and mandibular periosteum. Both structures were also visualized through orthogonal polarization spectral imaging and fluorescence confocal laser scanning microscopy. RESULTS: The microcirculatory variables in the mandibular periosteum proved similar to those in the tibia, indicating that no microcirculatory failure resulted from the exposure technique. CONCLUSION: This novel surgical approach provides simple access to the mandibular periosteum of the rat, offering an excellent opportunity for investigations of microcirculatory manifestations of dentoalveolar and maxillofacial diseases. This article is protected by copyright. All rights reserved. |
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| Terjedelem/Fizikai jellemzők: | 524-531 |
| ISSN: | 1073-9688 |