Development of a small-animal focal brain irradiation model to study radiation injury and radiation-injury modifiers
Abstract Purpose: Our aim was to establish an effective small-animal focal brain radiation model for research on brain injuries. Material and methods: Groups of up to six rats were exposed to a range of doses from 120-40 Gy, at 10 intervals of a 6 MeV electron beam. Open-field motor functions and w...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2013
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| Sorozat: | INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
89 No. 8 |
| doi: | 10.3109/09553002.2013.784424 |
| mtmt: | 2384738 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/12923 |
| Tartalmi kivonat: | Abstract Purpose: Our aim was to establish an effective small-animal focal brain radiation model for research on brain injuries. Material and methods: Groups of up to six rats were exposed to a range of doses from 120-40 Gy, at 10 intervals of a 6 MeV electron beam. Open-field motor functions and water maze learning-memory tests were performed after the irradiation at two-week intervals. Morphological changes were detected through repeated magnetic resonance imaging (MRI) monthly and were compared with the histopathological findings to determine if they predicted late microscopic changes. Results: The development of necrosis proved to be dose-dependent. 120 Gy resulted in serious deterioration within 4 weeks in all rats. Localized necrosis in one hemisphere was detected 2 months after the irradiation with >/= 70 Gy, and 3 months after 40-60 Gy consistent for all animals. The Morris water maze (MWM) tests proved to be the most sensitive tool for the early detection of a brain functional impairment. MRI screening provided useful information on the development of radiation necrosis, which defined the time point for histological examinations. Conclusions: The described method permits accurate dose delivery to a definite part in one hemisphere of the brain for six rats at a time. Following complex examinations, a dose of 40 Gy and a follow-up time of 4 months are proposed for investigations on neuroradiation modifiers. |
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| Terjedelem/Fizikai jellemzők: | 645-655 |
| ISSN: | 0955-3002 |