Comparative distribution of somatostatin and thrittene bioactive peptides in the central nervous system of rat measured by radioimmunoassay

In the present paper the development and application of a novel somatostatin RIA are described. 125I-labeling of Tyr(0)-somatostatin-14 (tyrosin(0)-somatostatin-14) was performed by the iodogen-method and the mono-iodinated peptide was sepd. by reversed-phase HPLC. As a practical application of the...

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Bibliographic Details
Main Authors: Fürjes G.
Lelesz Beáta
Tóth Gábor
Arday A.
Szilvássy Zoltán
Varga Angelika
Berényi E.
Németh J.
Format: Article
Published: 2017
Series:JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY 311 No. 3
doi:10.1007/s10967-016-5132-7

mtmt:3192960
Online Access:http://publicatio.bibl.u-szeged.hu/12227
Description
Summary:In the present paper the development and application of a novel somatostatin RIA are described. 125I-labeling of Tyr(0)-somatostatin-14 (tyrosin(0)-somatostatin-14) was performed by the iodogen-method and the mono-iodinated peptide was sepd. by reversed-phase HPLC. As a practical application of the novel RIA, the present work measured the somatostatin concn. parallel with the detn. of thrittene content in different rat brain areas. The highest somatostatin concns. were detected in the cerebellum. The thrittene immunoreactivity in the same brain parts showed a similar tendency. In the present study concluded that thrittene is not created via a specific biosynthetical route, but from the pre-prosomatostatin. [on SciFinder(R)]
Physical Description:1741-1749
ISSN:0236-5731