Comparative distribution of somatostatin and thrittene bioactive peptides in the central nervous system of rat measured by radioimmunoassay
In the present paper the development and application of a novel somatostatin RIA are described. 125I-labeling of Tyr(0)-somatostatin-14 (tyrosin(0)-somatostatin-14) was performed by the iodogen-method and the mono-iodinated peptide was sepd. by reversed-phase HPLC. As a practical application of the...
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| Format: | Article |
| Published: |
2017
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| Series: | JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
311 No. 3 |
| doi: | 10.1007/s10967-016-5132-7 |
| mtmt: | 3192960 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/12227 |
| Summary: | In the present paper the development and application of a novel somatostatin RIA are described. 125I-labeling of Tyr(0)-somatostatin-14 (tyrosin(0)-somatostatin-14) was performed by the iodogen-method and the mono-iodinated peptide was sepd. by reversed-phase HPLC. As a practical application of the novel RIA, the present work measured the somatostatin concn. parallel with the detn. of thrittene content in different rat brain areas. The highest somatostatin concns. were detected in the cerebellum. The thrittene immunoreactivity in the same brain parts showed a similar tendency. In the present study concluded that thrittene is not created via a specific biosynthetical route, but from the pre-prosomatostatin. [on SciFinder(R)] |
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| Physical Description: | 1741-1749 |
| ISSN: | 0236-5731 |