Liquid and subcritical fluid chromatographic enantioseparation of Nα-Fmoc proteinogenic amino acids on Quinidine-based zwitterionic and anion-exchanger type chiral stationary phases. A comparative study.
Stereoselective high-performance liq. chromatog. and subcrit. fluid chromatog. sepns. of 19 Nα-Fmoc proteinogenic amino acid enantiomers were carried out by using Quinidine-based zwitterionic and anion-exchanger-type chiral stationary phases Chiralpak ZWIX(-) and QD-AX. For optimization of retentio...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
Wiley-Liss
2017
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| Sorozat: | CHIRALITY: THE PHARMACOLOGICAL BIOLOGICAL AND CHEMICAL CONSEQUENCES OF MOLECULAR ASYMMETRY
29 No. 6 |
| doi: | 10.1002/chir.22700 |
| mtmt: | 3244835 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/12224 |
| Tartalmi kivonat: | Stereoselective high-performance liq. chromatog. and subcrit. fluid chromatog. sepns. of 19 Nα-Fmoc proteinogenic amino acid enantiomers were carried out by using Quinidine-based zwitterionic and anion-exchanger-type chiral stationary phases Chiralpak ZWIX(-) and QD-AX. For optimization of retention and enantioselectivity, the ratio of bulk solvent components (MeOH/MeCN, H2O/MeOH, or CO2/MeOH) and the nature and concn. of the acid and base additives (counter- and co-ions) were systematically varied. The effect of column temp. on the enantiosepn. was investigated and thermodn. parameters were calcd. from the van't Hoff plots ln α vs. 1/T. The thermodn. parameters revealed that the enantiosepns. were enthalpy-driven. The elution sequence was detd. in all cases and with the exception of Fmoc-Cys(Trt)-OH, it was identical on both chiral stationary phases whereby the L-enantiomers eluted before the D-enantiomers. [on SciFinder(R)] |
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| Terjedelem/Fizikai jellemzők: | 225-238 |
| ISSN: | 0899-0042 |