Regional differences in the neuronal expression of cyclooxygenase-2 (COX-2) in the newborn pig brain
Cycoxygene (COX-2 is the major costitutively expressed COX isoform in the newborn brain. COX-2 derived prostanoids and reactive oxygen species appear to play a major role in the mechanism of perinatal hypoxic-ischemic injury in the newborn piglet, an accepted animal model of the human term neonate....
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Main Authors: | |
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Format: | Article |
Published: |
2012
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Series: | ACTA HISTOCHEMICA ET CYTOCHEMICA
45 No. 3 |
doi: | 10.1267/ahc.11056 |
mtmt: | 2027259 |
Online Access: | http://publicatio.bibl.u-szeged.hu/11403 |
Summary: | Cycoxygene (COX-2 is the major costitutively expressed COX isoform in the newborn brain. COX-2 derived prostanoids and reactive oxygen species appear to play a major role in the mechanism of perinatal hypoxic-ischemic injury in the newborn piglet, an accepted animal model of the human term neonate. The study aimed to quantitatively determine COX-2 immunopositive neurons in different brain regions in piglets under normoxic conditions (n=15), and 4 hours after 10 min asphyxia (n=11). Asphyxia did not induce significant changes in neuronal COX-2 expression of any studied brain areas. In contrast, there was a marked regional difference in all experimental groups. Thus, significant difference was observed between fronto-parietal and temporo-occipital regions: 59±4% and 67±3% versus 41±2%* and 31±3%* respectively (mean±SEM, data are pooled from all subjects, n=26, *p<0.05, vs. fronto-parietal region). In the hippocampus, COX-2 immunopositivity was rare (highest expression in CA1 region: 14±2%). The studied subcortical areas showed negligible COX-2 staining. Our findings suggest that asphyxia does not significantly alter the pattern of neuronal COX-2 expression in the early reventilation period. Furthermore, based on the striking differences observed in cortical neuronal COX-2 distribution, the contribution of COX-2 mediated neuronal injury after asphyxia may also show region-specific differences. © 2012 The Japan Society of Histochemistry and Cytochemistry. |
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Physical Description: | 187-192 |
ISSN: | 0044-5991 |