Effects of chronic and subtoxic chlorobenzenes on adrenocorticotrophic hormone release

Many environmental chemicals and pesticides have been found to alter neuroendocrine communication in exposed biological objects. The environmental loads have primary and secondary effects that can alter the homeostatic regulation potential. Since it is difficult to avoid human exposition, a potentia...

Teljes leírás

Elmentve itt :
Bibliográfiai részletek
Szerzők: Molnár Zsolt
Pálföldi Regina
László Anna
Radács Marianna
Sepp Krisztián
Hausinger Péter
Tiszlavicz László
Valkusz Zsuzsanna
Gálfi Márta
Dokumentumtípus: Cikk
Megjelent: Elsevier 2015
Sorozat:JOURNAL OF ENVIRONMENTAL SCIENCES 34
doi:10.1016/j.jes.2015.03.014

mtmt:2934331
Online Access:http://publicatio.bibl.u-szeged.hu/10161
Leíró adatok
Tartalmi kivonat:Many environmental chemicals and pesticides have been found to alter neuroendocrine communication in exposed biological objects. The environmental loads have primary and secondary effects that can alter the homeostatic regulation potential. Since it is difficult to avoid human exposition, a potentially important area of research to develop in vivo and in vitro experimental models. In this context, the primary aim of this study was to demonstrate the effects of chlorobenzenes on adrenocorticotrophic hormone (ACTH) release. In our experimental study, male Wistar rats were exposed to 0.1, 1.0 and 10mug/b.w. (body weight)kg of 1,2,4- trichlorobenzene and hexachlorobenzene (ClB) mix via gastric tube for 30, 60 or 90days. At the endpoints of the experiment blood samples were taken and animals were decapitated. Primary, monolayer adenohypophysis cell cultures were prepared by enzymatic and mechanical digestion. The ACTH hormone content in serum and supernatant media was measured by immuno-chemiluminescence assay. The Mg(2+)-dependent ATPase activity was determined by modified method of Martin and Dotty. Significant differences were detected in the hormone release between the control and treated groups. The hormone release was enhanced characteristically in exposed groups depending upon the dose and duration of exposure. The Mg(2+)-ATPase activity enhanced after chronic and subtoxic ClB exposition. Light microscopy revealed that the adenohypophysis seemed to be more abundant. Results indicate that Wistar rats exposed to subtoxic ClB have direct and indirect effects on hypothalamus-hypophysis-adrenal axis.
Terjedelem/Fizikai jellemzők:165-170
ISSN:1001-0742