Identification and structural characterization of chondroitin sulfate disaccharides in human biglycan by nanoelectrospray ionization-ion mobility tandem mass spectrometry
We have implemented a superior glycomics method based on ion mobility separation (IMS) mass spectrometry (MS) and tandem MS (MS/MS) to characterize the chondoritin sulfate (CS) disaccharide domains in human biglycan (BGN). The high separation efficiency and sensitivity of IMS MS technique allowed th...
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| Dokumentumtípus: | Könyv része |
| Megjelent: |
University of Szeged
Szeged
2024
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| Sorozat: | Proceedings of the International Symposium on Analytical and Environmental Problems
30 |
| Kulcsszavak: | Biokémia, Tömegspektrometria |
| Tárgyszavak: | |
| Online Access: | http://acta.bibl.u-szeged.hu/85719 |
| Tartalmi kivonat: | We have implemented a superior glycomics method based on ion mobility separation (IMS) mass spectrometry (MS) and tandem MS (MS/MS) to characterize the chondoritin sulfate (CS) disaccharide domains in human biglycan (BGN). The high separation efficiency and sensitivity of IMS MS technique allowed the discrimination of five distinct CS motifs, of which four irregulated in their sulfation pattern. The structural investigation by IMS MS/MS disclosed that in one or both of the CS/DS chains, the non-reducing end is 3-O-sulfated GlcA in a rare bisulfated motif having the structure 3-O-sulfated -GlcA-4-O-sulfated GalNAc. Considering the role played by BGN in cancer cell spreading, the influence on this process of the newly identified sequences is to be investigated in the future. |
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| Terjedelem/Fizikai jellemzők: | 223-224 |
| ISBN: | 978-963-688-009-5 |