Enzyme-linked fluorescent immunoassay for monitoring the herbicide active ingredient glyphosate in water
Within a modular water quality assessment fluorimeter instrument family, a newly developed enzyme-linked fluorescent immunoassay has been utilized for the quantitative analytical measurement of the herbicide active ingredient glyphosate in surface water. The developed 96- well microplate-based compe...
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Dokumentumtípus: | Könyv része |
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2022
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Sorozat: | Proceedings of the International Symposium on Analytical and Environmental Problems
28 |
Kulcsszavak: | Analitikai kémia |
Tárgyszavak: | |
Online Access: | http://acta.bibl.u-szeged.hu/78561 |
LEADER | 02315naa a2200337 i 4500 | ||
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001 | acta78561 | ||
005 | 20230324065022.0 | ||
008 | 230324s2022 hu o 1|| eng d | ||
020 | |a 978-963-306-904-2 | ||
040 | |a SZTE Egyetemi Kiadványok Repozitórium |b hun | ||
041 | |a eng | ||
100 | 1 | |a Takács Eszter | |
245 | 1 | 0 | |a Enzyme-linked fluorescent immunoassay for monitoring the herbicide active ingredient glyphosate in water |h [elektronikus dokumentum] / |c Takács Eszter |
260 | |c 2022 | ||
300 | |a 350-354 | ||
490 | 0 | |a Proceedings of the International Symposium on Analytical and Environmental Problems |v 28 | |
520 | 3 | |a Within a modular water quality assessment fluorimeter instrument family, a newly developed enzyme-linked fluorescent immunoassay has been utilized for the quantitative analytical measurement of the herbicide active ingredient glyphosate in surface water. The developed 96- well microplate-based competitive immunoassay with fluorescence detection provides a 2.5- fold lower limit of detection (LOD = 0.09 ng/mL) in the investigated concentration range of glyphosate (0–100 ng/mL) compared to the detection of visual absorbance signals. Additionally, fluorescence detection resulted in a wider dynamic range for glyphosate measurement. Matrix effect was not observed for the undiluted surface water samples, and cross-reaction was not detected between glyphosate and its main metabolite (Naminomethylphosphonic acid) and structurally similar compounds. The method allows rapid monitoring of glyphosate as a ubiquitous water contaminant of agricultural origin that can affect, due to its global use, both aquatic and terrestrial ecosystems. | |
650 | 4 | |a Természettudományok | |
650 | 4 | |a Kémiai tudományok | |
695 | |a Analitikai kémia | ||
700 | 0 | 1 | |a Gémes Borbála |e aut |
700 | 0 | 1 | |a Szendrei Fanni |e aut |
700 | 0 | 1 | |a Keszei Csaba |e aut |
700 | 0 | 1 | |a Barócsi Attila |e aut |
700 | 0 | 1 | |a Lenk Sándor |e aut |
700 | 0 | 1 | |a Domján László |e aut |
700 | 0 | 1 | |a Mörtl Mária |e aut |
700 | 0 | 1 | |a Klátyik Szandra |e aut |
700 | 0 | 1 | |a Székács András |e aut |
711 | |a 28th International Symposium on Analytical and Environmental Problems |c Szeged |d 2022. november 14-15. | ||
856 | 4 | 0 | |u http://acta.bibl.u-szeged.hu/78561/1/proceedings_of_isaep_2022_350-354.pdf |z Dokumentum-elérés |