Enzyme-linked fluorescent immunoassay for monitoring the herbicide active ingredient glyphosate in water

Within a modular water quality assessment fluorimeter instrument family, a newly developed enzyme-linked fluorescent immunoassay has been utilized for the quantitative analytical measurement of the herbicide active ingredient glyphosate in surface water. The developed 96- well microplate-based compe...

Teljes leírás

Elmentve itt :
Bibliográfiai részletek
Szerzők: Takács Eszter
Gémes Borbála
Szendrei Fanni
Keszei Csaba
Barócsi Attila
Lenk Sándor
Domján László
Mörtl Mária
Klátyik Szandra
Székács András
Testületi szerző: 28th International Symposium on Analytical and Environmental Problems
Dokumentumtípus: Könyv része
Megjelent: 2022
Sorozat:Proceedings of the International Symposium on Analytical and Environmental Problems 28
Kulcsszavak:Analitikai kémia
Tárgyszavak:
Online Access:http://acta.bibl.u-szeged.hu/78561
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040 |a SZTE Egyetemi Kiadványok Repozitórium  |b hun 
041 |a eng 
100 1 |a Takács Eszter 
245 1 0 |a Enzyme-linked fluorescent immunoassay for monitoring the herbicide active ingredient glyphosate in water  |h [elektronikus dokumentum] /  |c  Takács Eszter 
260 |c 2022 
300 |a 350-354 
490 0 |a Proceedings of the International Symposium on Analytical and Environmental Problems  |v 28 
520 3 |a Within a modular water quality assessment fluorimeter instrument family, a newly developed enzyme-linked fluorescent immunoassay has been utilized for the quantitative analytical measurement of the herbicide active ingredient glyphosate in surface water. The developed 96- well microplate-based competitive immunoassay with fluorescence detection provides a 2.5- fold lower limit of detection (LOD = 0.09 ng/mL) in the investigated concentration range of glyphosate (0–100 ng/mL) compared to the detection of visual absorbance signals. Additionally, fluorescence detection resulted in a wider dynamic range for glyphosate measurement. Matrix effect was not observed for the undiluted surface water samples, and cross-reaction was not detected between glyphosate and its main metabolite (Naminomethylphosphonic acid) and structurally similar compounds. The method allows rapid monitoring of glyphosate as a ubiquitous water contaminant of agricultural origin that can affect, due to its global use, both aquatic and terrestrial ecosystems. 
650 4 |a Természettudományok 
650 4 |a Kémiai tudományok 
695 |a Analitikai kémia 
700 0 1 |a Gémes Borbála  |e aut 
700 0 1 |a Szendrei Fanni  |e aut 
700 0 1 |a Keszei Csaba  |e aut 
700 0 1 |a Barócsi Attila  |e aut 
700 0 1 |a Lenk Sándor  |e aut 
700 0 1 |a Domján László  |e aut 
700 0 1 |a Mörtl Mária  |e aut 
700 0 1 |a Klátyik Szandra  |e aut 
700 0 1 |a Székács András  |e aut 
711 |a 28th International Symposium on Analytical and Environmental Problems  |c Szeged  |d 2022. november 14-15. 
856 4 0 |u http://acta.bibl.u-szeged.hu/78561/1/proceedings_of_isaep_2022_350-354.pdf  |z Dokumentum-elérés