Enzyme-linked fluorescent immunoassay for monitoring the herbicide active ingredient glyphosate in water
Within a modular water quality assessment fluorimeter instrument family, a newly developed enzyme-linked fluorescent immunoassay has been utilized for the quantitative analytical measurement of the herbicide active ingredient glyphosate in surface water. The developed 96- well microplate-based compe...
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| Dokumentumtípus: | Könyv része |
| Megjelent: |
2022
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| Sorozat: | Proceedings of the International Symposium on Analytical and Environmental Problems
28 |
| Kulcsszavak: | Analitikai kémia |
| Tárgyszavak: | |
| Online Access: | http://acta.bibl.u-szeged.hu/78561 |
| Tartalmi kivonat: | Within a modular water quality assessment fluorimeter instrument family, a newly developed enzyme-linked fluorescent immunoassay has been utilized for the quantitative analytical measurement of the herbicide active ingredient glyphosate in surface water. The developed 96- well microplate-based competitive immunoassay with fluorescence detection provides a 2.5- fold lower limit of detection (LOD = 0.09 ng/mL) in the investigated concentration range of glyphosate (0–100 ng/mL) compared to the detection of visual absorbance signals. Additionally, fluorescence detection resulted in a wider dynamic range for glyphosate measurement. Matrix effect was not observed for the undiluted surface water samples, and cross-reaction was not detected between glyphosate and its main metabolite (Naminomethylphosphonic acid) and structurally similar compounds. The method allows rapid monitoring of glyphosate as a ubiquitous water contaminant of agricultural origin that can affect, due to its global use, both aquatic and terrestrial ecosystems. |
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| Terjedelem/Fizikai jellemzők: | 350-354 |
| ISBN: | 978-963-306-904-2 |