Action of Ganoderma lucidum mycelial growth filtrates on Erysiphe dffusa and embryotoxicity assessment in a chicken embryo model
This work aimed to evaluate the antimicrobial effect of Ganoderma lucidum mycelial growth filtrates (MGF) on the phytopathogen Erysiphe diffusa and their potential effects on the embryonic development of Gallus gallus. The antimicrobial activity was evaluated on E. diffusa spores by the microdilution...
Elmentve itt :
| Szerzők: | |
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| Dokumentumtípus: | Cikk |
| Megjelent: |
University of Szeged
Szeged
2021
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| Sorozat: | Acta biologica Szegediensis
65 No. 1 |
| Kulcsszavak: | Laboratóriumi technika - biológia |
| Tárgyszavak: | |
| doi: | 10.14232/abs.2021.1.47-57 |
| Online Access: | http://acta.bibl.u-szeged.hu/75579 |
| LEADER | 02345nab a2200289 i 4500 | ||
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| 005 | 20220512084005.0 | ||
| 008 | 220512s2021 hu o 0|| eng d | ||
| 022 | |a 1588-4082 | ||
| 024 | 7 | |a 10.14232/abs.2021.1.47-57 |2 doi | |
| 040 | |a SZTE Egyetemi Kiadványok Repozitórium |b hun | ||
| 041 | |a eng | ||
| 100 | 1 | |a Cruz Mycheli P. da | |
| 245 | 1 | 0 | |a Action of Ganoderma lucidum mycelial growth filtrates on Erysiphe dffusa and embryotoxicity assessment in a chicken embryo model |h [elektronikus dokumentum] / |c Cruz Mycheli P. da |
| 260 | |a University of Szeged |b Szeged |c 2021 | ||
| 300 | |a 47-57 | ||
| 490 | 0 | |a Acta biologica Szegediensis |v 65 No. 1 | |
| 520 | 3 | |a This work aimed to evaluate the antimicrobial effect of Ganoderma lucidum mycelial growth filtrates (MGF) on the phytopathogen Erysiphe diffusa and their potential effects on the embryonic development of Gallus gallus. The antimicrobial activity was evaluated on E. diffusa spores by the microdilution broth method. To evaluate embryotoxic and teratogenic effects, fertile eggs of G. gallus received injections of solutions containing the filtrates of G. lucidum through the air chamber. After three days of incubation, we opened the eggs and evaluated egg viability, embryo survival, malformation occurrence, embryonic staging and heart rate. Live embryos were prepared using whole mount technique and the morphological analysis was performed. We used the generalized linear model to fit embryotoxicity and teratogenicity data. We verified that G. lucidum MGF showed inhibitory activity in vitro against E. diffusa and the minimum inhibitory concentrations ranged from 5 to 10 mg/mL. We could also observe that the filtrates did not present embryotoxic or teratogenic effects on the early embryonic development of G. gallus, but induced significant differences in the embryonic mean heart rate and on the stage of embryonic development. | |
| 650 | 4 | |a Természettudományok | |
| 650 | 4 | |a Biológiai tudományok | |
| 695 | |a Laboratóriumi technika - biológia | ||
| 700 | 0 | 1 | |a Larentis Lucas T. |e aut |
| 700 | 0 | 1 | |a Vismara Edgar de S. |e aut |
| 700 | 0 | 1 | |a Vismara Lilian de S. |e aut |
| 700 | 0 | 1 | |a Freitas Patricia F. de |e aut |
| 700 | 0 | 1 | |a Mazaro Sérgio M. |e aut |
| 856 | 4 | 0 | |u http://acta.bibl.u-szeged.hu/75579/1/biologica_065_numb_001_047-057.pdf |z Dokumentum-elérés |