Determination of glutamate and gaba from rat central nervous system samples with HPLC utilizing fluorescent detection
The research of neurological diseases has great importance and the determination of neurotransmitter concentrations is essential during these investigations. The levels of main excitatory (glutamate) and inhibitory (γ-amino-butyric acid (GABA)) neurotransmitters often change as a result of pathologi...
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| Dokumentumtípus: | Könyv része |
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2019
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| Sorozat: | Proceedings of the International Symposium on Analytical and Environmental Problems
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| Kulcsszavak: | Ideggyógyászat - kutatás, Gyógyszerkémia |
| Online Access: | http://acta.bibl.u-szeged.hu/64899 |
| Tartalmi kivonat: | The research of neurological diseases has great importance and the determination of neurotransmitter concentrations is essential during these investigations. The levels of main excitatory (glutamate) and inhibitory (γ-amino-butyric acid (GABA)) neurotransmitters often change as a result of pathological alterations. For example, in case of migraine, elevated glutamate release is suggested to be a part of the pathomechanism, causing hypersensitivity to pain. Our goal in this study was to optimize a liquid chromatography method to measure glutamate and GABA from the trigeminal nucleus pars caudalis (TNC) of rats, which is responsible for pain processing. As a result, we were able to validate our method according to international guidelines where the investigated parameters were LOD, LOQ, precision and recovery. Furthermore, we applied a new internal standard which has not been published so far. This method will be utilized in the investigation of migraine animal models to evaluate potential new therapeutic approaches. |
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| Terjedelem/Fizikai jellemzők: | 427-431 |
| ISBN: | 978-963-306-702-4 |