Expression and production optimization of the cationic antimicrobial peptide indolicidin by the recombinant E. coli C41 (DE3) clones /
The cytoplasmic granules of bovine neutrophils naturally possess indolicidin - a promising cationic antimicrobial peptide as it displays inherent inhibitory activities against a broad type of microbial pathogens. In this study, a shake flask level production and expression optimizations of the indol...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
University of Szeged
Szeged
2018
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| Sorozat: | Acta biologica Szegediensis
62 No. 1 |
| Kulcsszavak: | Mikrobiológia |
| doi: | 10.14232/abs.2018.1.61-66 |
| Online Access: | http://acta.bibl.u-szeged.hu/58199 |
| Tartalmi kivonat: | The cytoplasmic granules of bovine neutrophils naturally possess indolicidin - a promising cationic antimicrobial peptide as it displays inherent inhibitory activities against a broad type of microbial pathogens. In this study, a shake flask level production and expression optimizations of the indolicidin by the recombinant Escherichia coli C41 (DE3) clones (transformed with pET21a(+) plasmid carrying indolicidin gene) were carried out under standard conditions, as to determine the conditions required for maximal production. It was determined that a concentration of 1 mM of IPTG was effective, the 2×YT with salts and LB media at pH 7.5 with 3-6 h of incubation were required for maximal indolicidin expression. |
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| Terjedelem/Fizikai jellemzők: | 61-66 |
| ISSN: | 1588-4082 |