Development of redoxprotein-based biohybrid sensor device
We have successfully developed an enzyme electrode based on photosynthetic reaction centre (RC) protein of Rhodobacter sphaeroides purple bacteria. We bound the protein chemically to the electrode surface through a layer of multi-walled carbon nanotubes (MWCNTs). The surface was borosilicate glass c...
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| Dokumentumtípus: | Könyv része |
| Megjelent: |
2017
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| Sorozat: | Proceedings of the International Symposium on Analytical and Environmental Problems
23 |
| Kulcsszavak: | Kémia, Elektrokémia, Fizikai kémia |
| Online Access: | http://acta.bibl.u-szeged.hu/56151 |
| Tartalmi kivonat: | We have successfully developed an enzyme electrode based on photosynthetic reaction centre (RC) protein of Rhodobacter sphaeroides purple bacteria. We bound the protein chemically to the electrode surface through a layer of multi-walled carbon nanotubes (MWCNTs). The surface was borosilicate glass covered with indium-tin-oxide (ITO) for its transparency and well-known qualities as an electric semiconductor. We achieved a bio-nanocomposite with good features to support the protein’s photosynthetic activity. To test it, we used the nanocomposite as a working electrode in a traditional electrochemical cell with electrolytes containing only mediators or mediators and inhibitors (herbicides that block the protein’s activity specifically). Our measurements provided great results in terms of electric current (“photocurrent”) generated by the photon-excited RC, regeneration of the electrode surface and stability of the composite. For further optimization of the system, we decided to replace the RC/MWCNT part with a two-component protein complex of chemically bound RC and cytochrome c, which is a natural electron donor of the RC protein. We have successfully prepared the complex and made several measurements to determine the quantity and quality of the product, with good results. |
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| Terjedelem/Fizikai jellemzők: | 36-41 |
| ISBN: | 978-963-306-563-1 |