Humán szérum tokoferol- és ubikinonszintek bioanalitikai vizsgálata
The vitamin E group of compounds and ubiquinone are widely known substances with antioxidant properties. However, the setup of high-performance liquid chromatography (HPLC) methods for the detection of these molecules in human serum are often difficult. We report here a reversed-phase HPLC method us...
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| Dokumentumtípus: | Könyv része |
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2015
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| Sorozat: | Proceedings of the International Symposium on Analytical and Environmental Problems
21 |
| Kulcsszavak: | Analitikai kémia |
| Online Access: | http://acta.bibl.u-szeged.hu/55991 |
| Tartalmi kivonat: | The vitamin E group of compounds and ubiquinone are widely known substances with antioxidant properties. However, the setup of high-performance liquid chromatography (HPLC) methods for the detection of these molecules in human serum are often difficult. We report here a reversed-phase HPLC method using diode array detection and a single C18 column separation kept at 25 °C with an isocratic system of acetonitrile, tetrahydrofuran, methanol, ammonium acetate and water at a flow-rate of 2.1 mL/min. This method resolves 3 tocopherols (α, γ, δ) and ubiquinone from human serum within 17 minutes with the application of tocol as an internal standard. We conclude that our procedure provides an adequate sample preparation and separation of the 3 examined tocopherols (α, γ, δ). Although the developed method has been proven to be efficient, accurate and inexpensive, further improvements in the quantitative assessment of ubiquinone are necessary. |
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| Terjedelem/Fizikai jellemzők: | 316-319 |
| ISBN: | 978-963-306-411-5 |