Indirect shoot organogenesis and plantlets regeneration from stem of ornamental Dieffenbachia maculata cv. Marianna
The present study reports a simple protocol for indirect shoot organogenesis and plantlets regeneration of Dieffenbachia maculata cv. Marianna using stem segments from in vitro shoot culture. Different concentrations and combinations of indole-3-acetic acid (IAA), 1- naphthalene acetic acid (NAA), 2...
Elmentve itt :
| Szerzők: | |
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2007
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| Sorozat: | Acta biologica Szegediensis
51 No. 2 |
| Kulcsszavak: | Akklimatizálódás - kontyvirágfélék |
| Online Access: | http://acta.bibl.u-szeged.hu/55385 |
| Tartalmi kivonat: | The present study reports a simple protocol for indirect shoot organogenesis and plantlets regeneration of Dieffenbachia maculata cv. Marianna using stem segments from in vitro shoot culture. Different concentrations and combinations of indole-3-acetic acid (IAA), 1- naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2, 4-D), 6-benzyladenine (BA), and kinetin (Kin) were used for callus induction and shoot organogenesis. The frequency of callus formation reached 87% and the highest number of shoots per callus was 4.8 for explants cultured on Murashige and Skoog (1962) medium supplemented with 15 mg I'1 BA + 15 mg I'1 NAA. Shoots were further grown and rooted on MS hormone free medium. Regenerated plantlets were successfully acclimatized in greenhouse with 100% survival rate. |
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| Terjedelem/Fizikai jellemzők: | 113-116 |
| ISSN: | 1588-385X |