Species composition of trichoderma communities in Hungarian soils used for vegetable cultivation
Species of the genus Trichoderma are commonly found free-living fungi in soil and rootecosystems. It is known that the rhizosphere of agricultural soils is an ideal source of beneficial Trichoderma strains with biocontrol potential, as some of the strains showed excellent antagonistic abilities agai...
Elmentve itt :
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Dokumentumtípus: | Cikk |
Megjelent: |
2012
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Sorozat: | Review on agriculture and rural development
1 No. 1 |
Kulcsszavak: | Trichoderma, Talajvizsgálat - előadáskivonat, Rizoszféra - előadáskivonat, Zöldségtermesztés - Magyarország - előadáskivonat |
Tárgyszavak: | |
Online Access: | http://acta.bibl.u-szeged.hu/55232 |
Tartalmi kivonat: | Species of the genus Trichoderma are commonly found free-living fungi in soil and rootecosystems. It is known that the rhizosphere of agricultural soils is an ideal source of beneficial Trichoderma strains with biocontrol potential, as some of the strains showed excellent antagonistic abilities against plant pathogenic fungi. Others are able to improve plant growth, root in particular, promoting drought resistance in some crops. Biodiversity of Trichoderma isolates from the rizosphere of different vegetables (pepper, tomato, carrot, salad, spinach, pumpkin, cabbage, kohlrabi, parsley, celery, potato and bean) in garden soil samples collected at different locations in Hungary (Szeged-Sziksóstó, Balástya, Hódmezővásárhely, Szentes, Veszprém, Ózd) was comparatively examined during this study. Trichoderma strains were isolated directly from the chopped roots of the examined vegetables on dichloran - Rose Bengal medium. DNA isolation and PCR amplification of the internal transcribed spacer (ITS1-5.8S rDNA-ITS2) region have been used for the identification of the isolates and for the investigation of their biodiversity. Trichoderma isolates were identified based on their ITS sequences with the aid of the barcoding program TrichOKEY 2.0 available online at the home page of the International Subcommission on Trichoderma and Hypocrea Taxonomy (www.isth.info). Among the detected isolates, species known as promising biocontrol agents (T. harzianum, T. virens, T. atroviride, T. asperellum) could be identified. Data about the biodiversity of the genus Trichoderma in vegetable rhizosphere and surveying the in vitro antagonistic abilities of the isolated Trichoderma strains may reveal potential biocontrol agents against plant pathogenic fungi. |
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Terjedelem/Fizikai jellemzők: | 483 |
ISSN: | 2063-4803 |