01796nab a2200253 i 4500 publ5765 20220919085349.0 151102s2012 hu o 0|| zxx d 1545-9985 10.1038/nsmb.2224 doi 2537318 mtmt SZTE Publicatio Repozitórium hun zxx Pankotai Tibor DNAPKcs-dependent arrest of RNA polymerase II transcription in the presence of DNA breaks [elektronikus dokumentum] / Pankotai Tibor 2012-03 276-282 Nature structural & molecular biology 19 No. 3 DNA double-strand break (DSB) repair interferes with ongoing cellular processes, including replication and transcription. Although the process of replication stalling upon collision of replication forks with damaged DNA has been extensively studied, the fate of elongating RNA polymerase II (RNAPII) that encounters a DSB is not well understood. We show that the occurrence of a single DSB at a human RNAPII-transcribed gene leads to inhibition of transcription elongation and reinitiation. Upon inhibition of DNA protein kinase (DNAPK), RNAPII bypasses the break and continues transcription elongation, suggesting that it is not the break per se that inhibits the processivity of RNAPII, but the activity of DNAPK. We also show that the mechanism of DNAPK-mediated transcription inhibition involves the proteasome-dependent pathway. The results point to the pivotal role of DNAPK activity in the eviction of RNAPII from DNA upon encountering a DNA lesion. Biológiai tudományok Bonhomme Céline aut Chen David aut Soutoglou Evi aut http://publicatio.bibl.u-szeged.hu/5765/1/NSMB.pdf Dokumentum-elérés