Application of the immunoperoxidase technique to formalin fixed, paraffin embedded kidney biopsies

Different immunohistological methods were compared in 30 renal biopsy cases. The direct immunoperoxidase method gave identical results with immunofluorescence in 88% concerning IgG and C3, while IgA, IgM and fibrinogen have been identically detected by the PAP method in 82%. The non-identical result...

Teljes leírás

Elmentve itt :
Bibliográfiai részletek
Szerzők: Nagyné Kemény Éva Ilona
Iványi Béla
Németh Annamária
Sonkodi Sándor
Dokumentumtípus: Cikk
Megjelent: 1983
Sorozat:ZENTRALBLATT FÜR ALLGEMEINE PATHOLOGIE UND PATHOLOGISCHE ANATOMIE 128 No. 1-2
Tárgyszavak:
mtmt:1389021
Online Access:http://publicatio.bibl.u-szeged.hu/33281
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245 1 0 |a Application of the immunoperoxidase technique to formalin fixed, paraffin embedded kidney biopsies  |h [elektronikus dokumentum] /  |c  Nagyné Kemény Éva Ilona 
260 |c 1983 
300 |a 119-126 
490 0 |a ZENTRALBLATT FÜR ALLGEMEINE PATHOLOGIE UND PATHOLOGISCHE ANATOMIE  |v 128 No. 1-2 
520 3 |a Different immunohistological methods were compared in 30 renal biopsy cases. The direct immunoperoxidase method gave identical results with immunofluorescence in 88% concerning IgG and C3, while IgA, IgM and fibrinogen have been identically detected by the PAP method in 82%. The non-identical results of the direct method consisted, with one exception, of negative readings, while the non-identical PAP readings were positive with two exceptions. Thus the trypsin-immunoperoxidase method proved to be well applicable on paraffin embedded formalin fixed material. In 10 cases the HRP-labelled direct method was applied also to fresh, frozen sections. It gave almost entirely identical results to immunofluorescence. 
650 4 |a Általános orvostudomány 
700 0 1 |a Iványi Béla  |e aut 
700 0 1 |a Németh Annamária  |e aut 
700 0 1 |a Sonkodi Sándor  |e aut 
856 4 0 |u http://publicatio.bibl.u-szeged.hu/33281/1/aok_klny_412_83.pdf  |z Dokumentum-elérés