Dysregulated miRNA and mRNA Expression Affect Overlapping Pathways in a Huntington’s Disease Model
Huntington’s disease (HD) is a fatal neurodegenerative disorder caused by the expansion of a CAG trinucleotide repeat in the Huntingtin gene. Transcriptional dysregulation is one of the main cellular processes affected by mutant Huntingtin (mHtt). In this study, we investigate the alterations in miR...
Elmentve itt :
| Szerzők: | |
|---|---|
| Dokumentumtípus: | Cikk |
| Megjelent: |
2023
|
| Sorozat: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
24 No. 15 |
| Tárgyszavak: | |
| doi: | 10.3390/ijms241511942 |
| mtmt: | 34173172 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/29631 |
| LEADER | 02217nab a2200265 i 4500 | ||
|---|---|---|---|
| 001 | publ29631 | ||
| 005 | 20240222082742.0 | ||
| 008 | 240222s2023 hu o 0|| eng d | ||
| 022 | |a 1661-6596 | ||
| 024 | 7 | |a 10.3390/ijms241511942 |2 doi | |
| 024 | 7 | |a 34173172 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
| 041 | |a eng | ||
| 100 | 1 | |a Zsindely Nóra | |
| 245 | 1 | 0 | |a Dysregulated miRNA and mRNA Expression Affect Overlapping Pathways in a Huntington’s Disease Model |h [elektronikus dokumentum] / |c Zsindely Nóra |
| 260 | |c 2023 | ||
| 300 | |a 21 | ||
| 490 | 0 | |a INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |v 24 No. 15 | |
| 520 | 3 | |a Huntington’s disease (HD) is a fatal neurodegenerative disorder caused by the expansion of a CAG trinucleotide repeat in the Huntingtin gene. Transcriptional dysregulation is one of the main cellular processes affected by mutant Huntingtin (mHtt). In this study, we investigate the alterations in miRNA and mRNA expression levels in a Drosophila model of HD by RNA sequencing and assess the functional effects of misregulated miRNAs in vivo. We found that in head samples of HD flies, the level of 32 miRNAs changed significantly; half of these were upregulated, while the other half were downregulated. After comparing miRNA and mRNA expression data, we discovered similarities in the impacted molecular pathways. Additionally, we observed that the putative targets of almost all dysregulated miRNAs were overrepresented among the upregulated mRNAs. We tested the effects of overexpression of five misregulated miRNAs in the HD model and found that while mir-10 and mir-219 enhanced, mir-137, mir-305, and mir-1010 ameliorated mHtt-induced phenotypes. Based on our results, we propose that while altered expression of mir-10, mir-137, and mir-1010 might be part of HD pathology, the upregulation of mir-305 might serve as a compensatory mechanism as a response to mHtt-induced transcriptional dysregulation. | |
| 650 | 4 | |a Biológiai tudományok | |
| 700 | 0 | 1 | |a Nagy Gábor |e aut |
| 700 | 0 | 1 | |a Siági Fruzsina |e aut |
| 700 | 0 | 1 | |a Farkas Anita |e aut |
| 700 | 0 | 1 | |a Bodai László |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/29631/1/2023ZsindelyHDmiRNA.pdf |z Dokumentum-elérés |