The anti-inflammatory effect of dimethyl trisulfide in experimental acute pancreatitis

Various organosulfur compounds, such as dimethyl trisulfide (DMTS), display anti-inflammatory properties. We aimed to examine the effects of DMTS on acute pancreatitis (AP) and its mechanism of action in both in vivo and in vitro studies. AP was induced in FVB/n mice or Wistar rats by caerulein, eth...

Full description

Saved in:
Bibliographic Details
Main Authors: Orján Erik Márk
Kormányos Eszter Sára
Fűr Gabriella
Dombi Ágnes
Bálint Emese Réka
Balla Zsolt
Balog Beáta Adél
Dágó Ágnes
Totonji Ahmad
Bátai István Zoárd
Jurányi Eszter Petra
Ditrói Tamás
Al-omari Ammar
Pozsgai Gábor
Kormos Viktória
Nagy Péter
Pintér Erika
Rakonczay Zoltán
Kiss Lóránd
Format: Article
Published: 2023
Series:SCIENTIFIC REPORTS 13 No. 1
Subjects:
doi:10.1038/s41598-023-43692-9

mtmt:34183455
Online Access:http://publicatio.bibl.u-szeged.hu/28471
Description
Summary:Various organosulfur compounds, such as dimethyl trisulfide (DMTS), display anti-inflammatory properties. We aimed to examine the effects of DMTS on acute pancreatitis (AP) and its mechanism of action in both in vivo and in vitro studies. AP was induced in FVB/n mice or Wistar rats by caerulein, ethanol-palmitoleic acid, or L-ornithine-HCl. DMTS treatments were administered subcutaneously. AP severity was assessed by pancreatic histological scoring, pancreatic water content, and myeloperoxidase activity measurements. The behaviour of animals was followed. Pancreatic heat shock protein 72 (HSP72) expression, sulfide, and protein persulfidation were measured. In vitro acinar viability, intracellular Ca 2+ concentration, and reactive oxygen species production were determined. DMTS dose-dependently decreased the severity of AP. It declined the pancreatic infiltration of leukocytes and cellular damage in mice. DMTS upregulated the HSP72 expression during AP and elevated serum sulfide and low molecular weight persulfide levels. DMTS exhibited cytoprotection against hydrogen peroxide and AP-inducing agents. It has antioxidant properties and modulates physiological but not pathophysiological Ca 2+ signalling. Generally, DMTS ameliorated AP severity and protected pancreatic acinar cells. Our findings indicate that DMTS is a sulfur donor with anti-inflammatory and antioxidant effects, and organosulfur compounds require further investigation into this potentially lethal disease.
Physical Description:19
ISSN:2045-2322