Unravelling the Effects of Syndecan-4 Knockdown on Skeletal Muscle Functions
The remodelling of the extracellular matrix plays an important role in skeletal muscle development and regeneration. Syndecan-4 is a cell surface proteoglycan crucial for muscle differentiation. Syndecan-4−/− mice have been reported to be unable to regenerate following muscle damage. To investigate...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2023
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| Sorozat: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
24 No. 8 |
| Tárgyszavak: | |
| doi: | 10.3390/ijms24086933 |
| mtmt: | 33742741 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/28164 |
| LEADER | 02563nab a2200337 i 4500 | ||
|---|---|---|---|
| 001 | publ28164 | ||
| 005 | 20230904082642.0 | ||
| 008 | 230904s2023 hu o 0|| Angol d | ||
| 022 | |a 1661-6596 | ||
| 024 | 7 | |a 10.3390/ijms24086933 |2 doi | |
| 024 | 7 | |a 33742741 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
| 041 | |a Angol | ||
| 100 | 1 | |a Sztretye Mónika | |
| 245 | 1 | 0 | |a Unravelling the Effects of Syndecan-4 Knockdown on Skeletal Muscle Functions |h [elektronikus dokumentum] / |c Sztretye Mónika |
| 260 | |c 2023 | ||
| 300 | |a 19 | ||
| 490 | 0 | |a INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |v 24 No. 8 | |
| 520 | 3 | |a The remodelling of the extracellular matrix plays an important role in skeletal muscle development and regeneration. Syndecan-4 is a cell surface proteoglycan crucial for muscle differentiation. Syndecan-4−/− mice have been reported to be unable to regenerate following muscle damage. To investigate the consequences of the decreased expression of Syndecan-4, we have studied the in vivo and in vitro muscle performance and the excitation–contraction coupling machinery in young and aged Syndecan-4+/− (SDC4) mice. In vivo grip force was decreased significantly as well as the average and maximal speed of voluntary running in SDC4 mice, regardless of their age. The maximal in vitro twitch force was reduced in both EDL and soleus muscles from young and aged SDC4 mice. Ca2+ release from the sarcoplasmic reticulum decreased significantly in the FDB fibres of young SDC4 mice, while its voltage dependence was unchanged regardless of age. These findings were present in muscles from young and aged mice as well. On C2C12 murine skeletal muscle cells, we have also found altered calcium homeostasis upon Syndecan-4 silencing. The decreased expression of Syndecan-4 leads to reduced skeletal muscle performance in mice and altered motility in C2C12 myoblasts via altered calcium homeostasis. The altered muscle force performance develops at an early age and is maintained throughout the life course of the animal until old age. | |
| 650 | 4 | |a Biológiai tudományok | |
| 650 | 4 | |a Általános orvostudomány | |
| 700 | 0 | 1 | |a Singlár Zoltán |e aut |
| 700 | 0 | 1 | |a Ganbat Nyamkhuu |e aut |
| 700 | 0 | 2 | |a Al-Gaadi Dána |e aut |
| 700 | 0 | 2 | |a Szabó Kitti |e aut |
| 700 | 0 | 2 | |a Köhler Zoltán Márton |e aut |
| 700 | 0 | 2 | |a Dux László |e aut |
| 700 | 0 | 2 | |a Keller-Pintér Anikó |e aut |
| 700 | 0 | 2 | |a Csernoch László |e aut |
| 700 | 0 | 2 | |a Szentesi Péter |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/28164/1/ijms-24-06933.pdf |z Dokumentum-elérés |