Advantages of Multiplexing Ability of the Orbitrap Mass Analyzer in the Multi-Mycotoxin Analysis
In routine measurements, the length of the analysis time and nfumber of samples analysed during a time unit are crucial parameters, which are especially important for the food analysis, particularly in the case of mycotoxin determinations. High-resolution equipment, including time-of-flight or Orbit...
Elmentve itt :
| Szerzők: | |
|---|---|
| Dokumentumtípus: | Cikk |
| Megjelent: |
2023
|
| Sorozat: | TOXINS
15 No. 2 |
| Tárgyszavak: | |
| doi: | 10.3390/toxins15020134 |
| mtmt: | 33629517 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/28117 |
| LEADER | 02344nab a2200277 i 4500 | ||
|---|---|---|---|
| 001 | publ28117 | ||
| 005 | 20230829080946.0 | ||
| 008 | 230829s2023 hu o 0|| Angol d | ||
| 022 | |a 2072-6651 | ||
| 024 | 7 | |a 10.3390/toxins15020134 |2 doi | |
| 024 | 7 | |a 33629517 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
| 041 | |a Angol | ||
| 100 | 1 | |a Rakk Dávid | |
| 245 | 1 | 0 | |a Advantages of Multiplexing Ability of the Orbitrap Mass Analyzer in the Multi-Mycotoxin Analysis |h [elektronikus dokumentum] / |c Rakk Dávid |
| 260 | |c 2023 | ||
| 300 | |a 15 | ||
| 490 | 0 | |a TOXINS |v 15 No. 2 | |
| 520 | 3 | |a In routine measurements, the length of the analysis time and nfumber of samples analysed during a time unit are crucial parameters, which are especially important for the food analysis, particularly in the case of mycotoxin determinations. High-resolution equipment, including time-of-flight or Orbitrap analyzators, can provide stable instrumental background for high-throughput analyses. In this report, a short, 1 min MS-based multi-mycotoxin method was developed with the application of a short column as a reduced chromatographic separation, taking advantages of the multiplexing and high-resolution capability of the QExactive Orbitrap MS possessing sub-1 ppm mass accuracy. The performance of the method was evaluated regarding selectivity, LOD, LOQ, linearity, matrix effect, and recovery, and compared to a UHPLC-MS/MS method. The final multiplexing method was able to quantify 11 mycotoxins in defined ranges (aflatoxins (corn, 2.8–600 μg/kg; wheat, 1.5–350 μg/kg), deoxynivalenol (corn, 640–9600 μg/kg; wheat, 128–3500 μg/kg), fumonisins (corn, 20–1500 μg/kg; wheat, 30–3500 μg/kg), HT-2 (corn, 64–5200 μg/kg; wheat, 61–3500 μg/kg), T-2 (corn, 10–800 μg/kg; wheat, 4–250 μg/kg), ochratoxin (corn, 4.7–600 μg/kg; wheat, 1–1000 μg/kg), zearalenone (corn, 64–4800 μg/kg; wheat, 4–500 μg/kg)) within one minute in corn and wheat matrices at the MRL levels stated by the European Union. | |
| 650 | 4 | |a Biológiai tudományok | |
| 700 | 0 | 1 | |a Kukolya József |e aut |
| 700 | 0 | 1 | |a Škrbić Biljana D. |e aut |
| 700 | 0 | 1 | |a Vágvölgyi Csaba |e aut |
| 700 | 0 | 1 | |a Varga Mónika |e aut |
| 700 | 0 | 1 | |a Szekeres András |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/28117/1/toxins-15-00134.pdf |z Dokumentum-elérés |