Protein kinase c activation in aplysia neurons by phorbol diacetate comparison of effects following extracellular or intracellular application /
The effects of protein kinase C activation on electrophysiological phenomena of neurons in Aplysia californica ganglions were studied. The enzyme was activated by phorbol-12,13-diacetate applied either extracellularly by perfusion, or by intracellular pressure injection. In both forms of application...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
1993
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| Sorozat: | ACTA PHYSIOLOGICA HUNGARICA
81 No. 2 |
| Tárgyszavak: | |
| mtmt: | 1240484 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/27599 |
| Tartalmi kivonat: | The effects of protein kinase C activation on electrophysiological phenomena of neurons in Aplysia californica ganglions were studied. The enzyme was activated by phorbol-12,13-diacetate applied either extracellularly by perfusion, or by intracellular pressure injection. In both forms of application, an increase in the potential upstroke speed and amplitude as well as a reduction of the depolarization evoked by extracellular acetylcholine application was found. A protein kinase C blocker, H-7, had opposite effects on the action potential. All observed actions of the phorbol ester were consistently faster in intracellular application than extracellular. |
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| Terjedelem/Fizikai jellemzők: | 147-157 |
| ISSN: | 0231-424X |