Sensing photosynthetic herbicides in an electrochemical flow cell
Specific inhibitory reactions of herbicides with photosynthetic reaction centers bound to working electrodes were monitored in a conventional electrochemical cell and a newly designed microfluidic electrochemical flow cell. In both cases, the bacterial reaction centers were bound to a transparent co...
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Main Authors: | |
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Format: | Article |
Published: |
2017
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Series: | PHOTOSYNTHESIS RESEARCH
132 No. 2 |
Subjects: | |
doi: | 10.1007/s11120-016-0314-2 |
mtmt: | 3167402 |
Online Access: | http://publicatio.bibl.u-szeged.hu/27373 |
Summary: | Specific inhibitory reactions of herbicides with photosynthetic reaction centers bound to working electrodes were monitored in a conventional electrochemical cell and a newly designed microfluidic electrochemical flow cell. In both cases, the bacterial reaction centers were bound to a transparent conductive metal oxide, indium-tin-oxide, electrode through carbon nanotubes. In the conventional cell, photocurrent densities of up to a few muA/cm2 could be measured routinely. The photocurrent could be blocked by the photosynthetic inhibitor terbutryn (I 50 = 0.38 +/- 0.14 muM) and o-phenanthroline (I 50 = 63.9 +/- 12.2 muM). The microfluidic flow cell device enabled us to reduce the sample volume and to simplify the electrode arrangement. The useful area of the electrodes remained the same (ca. 2 cm2), similar to the classical electrochemical cell; however, the size of the cell was reduced considerably. The microfluidic flow control enabled us monitoring in real time the binding/unbinding of the inhibitor and cofactor molecules at the secondary quinone site. |
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Physical Description: | 127-134 |
ISSN: | 0166-8595 |