Sensing photosynthetic herbicides in an electrochemical flow cell

Specific inhibitory reactions of herbicides with photosynthetic reaction centers bound to working electrodes were monitored in a conventional electrochemical cell and a newly designed microfluidic electrochemical flow cell. In both cases, the bacterial reaction centers were bound to a transparent co...

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Bibliographic Details
Main Authors: Szabó Tibor
Csekő Richárd
Hajdu Kata
Nagy Krisztina
Sipos Orsolya
Galajda Péter
Garab Győző
Nagy László
Format: Article
Published: 2017
Series:PHOTOSYNTHESIS RESEARCH 132 No. 2
Subjects:
doi:10.1007/s11120-016-0314-2

mtmt:3167402
Online Access:http://publicatio.bibl.u-szeged.hu/27373
Description
Summary:Specific inhibitory reactions of herbicides with photosynthetic reaction centers bound to working electrodes were monitored in a conventional electrochemical cell and a newly designed microfluidic electrochemical flow cell. In both cases, the bacterial reaction centers were bound to a transparent conductive metal oxide, indium-tin-oxide, electrode through carbon nanotubes. In the conventional cell, photocurrent densities of up to a few muA/cm2 could be measured routinely. The photocurrent could be blocked by the photosynthetic inhibitor terbutryn (I 50 = 0.38 +/- 0.14 muM) and o-phenanthroline (I 50 = 63.9 +/- 12.2 muM). The microfluidic flow cell device enabled us to reduce the sample volume and to simplify the electrode arrangement. The useful area of the electrodes remained the same (ca. 2 cm2), similar to the classical electrochemical cell; however, the size of the cell was reduced considerably. The microfluidic flow control enabled us monitoring in real time the binding/unbinding of the inhibitor and cofactor molecules at the secondary quinone site.
Physical Description:127-134
ISSN:0166-8595