The Tryptophan-Kynurenine Metabolic System Is Suppressed in Cuprizone-Induced Model of Demyelination Simulating Progressive Multiple Sclerosis
Progressive multiple sclerosis (MS) is a chronic disease with a unique pattern, which is histologically classified into the subpial type 3 lesions in the autopsy. The lesion is also homologous to that of cuprizone (CPZ) toxin-induced animal models of demyelination. Aberration of the tryptophan (TRP)...
Elmentve itt :
| Szerzők: | |
|---|---|
| Dokumentumtípus: | Cikk |
| Megjelent: |
2023
|
| Sorozat: | BIOMEDICINES
11 No. 3 |
| Tárgyszavak: | |
| doi: | 10.3390/biomedicines11030945 |
| mtmt: | 33709302 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/26787 |
| LEADER | 02704nab a2200337 i 4500 | ||
|---|---|---|---|
| 001 | publ26787 | ||
| 005 | 20230322133513.0 | ||
| 008 | 230322s2023 hu o 0|| Angol d | ||
| 022 | |a 2227-9059 | ||
| 024 | 7 | |a 10.3390/biomedicines11030945 |2 doi | |
| 024 | 7 | |a 33709302 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
| 041 | |a Angol | ||
| 100 | 1 | |a Polyák Helga | |
| 245 | 1 | 4 | |a The Tryptophan-Kynurenine Metabolic System Is Suppressed in Cuprizone-Induced Model of Demyelination Simulating Progressive Multiple Sclerosis |h [elektronikus dokumentum] / |c Polyák Helga |
| 260 | |c 2023 | ||
| 300 | |a 20 | ||
| 490 | 0 | |a BIOMEDICINES |v 11 No. 3 | |
| 520 | 3 | |a Progressive multiple sclerosis (MS) is a chronic disease with a unique pattern, which is histologically classified into the subpial type 3 lesions in the autopsy. The lesion is also homologous to that of cuprizone (CPZ) toxin-induced animal models of demyelination. Aberration of the tryptophan (TRP)-kynurenine (KYN) metabolic system has been observed in patients with MS; nevertheless, the KYN metabolite profile of progressive MS remains inconclusive. In this study, C57Bl/6J male mice were treated with 0.2% CPZ toxin for 5 weeks and then underwent 4 weeks of recovery. We measured the levels of serotonin, TRP, and KYN metabolites in the plasma and the brain samples of mice at weeks 1, 3, and 5 of demyelination, and at weeks 7 and 9 of remyelination periods by ultra-high-performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS) after body weight measurement and immunohistochemical analysis to confirm the development of demyelination. The UHPLC-MS/MS measurements demonstrated a significant reduction of kynurenic acid, 3-hydoxykynurenine (3-HK), and xanthurenic acid in the plasma and a significant reduction of 3-HK, and anthranilic acid in the brain samples at week 5. Here, we show the profile of KYN metabolites in the CPZ-induced mouse model of demyelination. Thus, the KYN metabolite profile potentially serves as a biomarker of progressive MS and thus opens a new path toward planning personalized treatment, which is frequently obscured with immunologic components in MS deterioration. | |
| 650 | 4 | |a Klinikai orvostan | |
| 700 | 0 | 1 | |a Galla Zsolt |e aut |
| 700 | 0 | 1 | |a Nánási Nikolett |e aut |
| 700 | 0 | 1 | |a Cseh Edina Katalin |e aut |
| 700 | 0 | 1 | |a Rajda Cecília |e aut |
| 700 | 0 | 1 | |a Veres Gábor |e aut |
| 700 | 0 | 1 | |a Spekker Eleonóra |e aut |
| 700 | 0 | 1 | |a Szabó Ágnes |e aut |
| 700 | 0 | 1 | |a Klivényi Péter |e aut |
| 700 | 0 | 1 | |a Tanaka Masaru |e aut |
| 700 | 0 | 1 | |a Vécsei László |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/26787/1/PolyakH._thetryptophan-kynureninemetabolicsystem....pdf |z Dokumentum-elérés |