A complete nicotinate degradation pathway in the microbial eukaryote Aspergillus nidulans
Several strikingly different aerobic and anaerobic pathways of nicotinate breakdown are extant in bacteria. Here, through reverse genetics and analytical techniques we elucidated in Aspergillus nidulans, a complete eukaryotic nicotinate utilization pathway. The pathway extant in this fungus and othe...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2022
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| Sorozat: | COMMUNICATIONS BIOLOGY
5 No. 1 |
| Tárgyszavak: | |
| doi: | 10.1038/s42003-022-03684-3 |
| mtmt: | 33025855 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/24834 |
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| 024 | 7 | |a 10.1038/s42003-022-03684-3 |2 doi | |
| 024 | 7 | |a 33025855 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
| 041 | |a Angol | ||
| 100 | 1 | |a Bokor Eszter | |
| 245 | 1 | 2 | |a A complete nicotinate degradation pathway in the microbial eukaryote Aspergillus nidulans |h [elektronikus dokumentum] / |c Bokor Eszter |
| 260 | |c 2022 | ||
| 300 | |a Terjedelem: 11 p.-Azonosító: 723 | ||
| 490 | 0 | |a COMMUNICATIONS BIOLOGY |v 5 No. 1 | |
| 520 | 3 | |a Several strikingly different aerobic and anaerobic pathways of nicotinate breakdown are extant in bacteria. Here, through reverse genetics and analytical techniques we elucidated in Aspergillus nidulans, a complete eukaryotic nicotinate utilization pathway. The pathway extant in this fungus and other ascomycetes, is quite different from bacterial ones. All intermediate metabolites were identified. The cognate proteins, encoded by eleven genes (hxn) mapping in three clusters are co-regulated by a specific transcription factor. Several enzymatic steps have no prokaryotic equivalent and two metabolites, 3-hydroxypiperidine-2,6-dione and 5,6-dihydroxypiperidine-2-one, have not been identified previously in any organism, the latter being a novel chemical compound. Hydrolytic ring opening results in α-hydroxyglutaramate, a compound not detected in analogous prokaryotic pathways. Our earlier phylogenetic analysis of Hxn proteins together with this complete biochemical pathway illustrates convergent evolution of catabolic pathways between fungi and bacteria. | |
| 650 | 4 | |a Biológiai tudományok | |
| 700 | 0 | 1 | |a Ámon Judit |e aut |
| 700 | 0 | 1 | |a Varga Mónika |e aut |
| 700 | 0 | 1 | |a Szekeres András |e aut |
| 700 | 0 | 1 | |a Hegedüs Zsófia |e aut |
| 700 | 0 | 1 | |a Jakusch Tamás |e aut |
| 700 | 0 | 1 | |a Szakonyi Zsolt |e aut |
| 700 | 0 | 1 | |a Flipphi Michel Johannes Anthonie |e aut |
| 700 | 0 | 1 | |a Vágvölgyi Csaba |e aut |
| 700 | 0 | 1 | |a Gácser Attila |e aut |
| 700 | 0 | 1 | |a Scazzocchio Claudio |e aut |
| 700 | 0 | 2 | |a Kozma-Bognárné Hamari Zsuzsanna |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/24834/1/s42003-022-03684-3.pdf |z Dokumentum-elérés |