Novel Lysine-Rich Delivery Peptides of Plant Origin ERD and Human S100 The Effect of Carboxyfluorescein Conjugation, Influence of Aromatic and Proline Residues, Cellular Internalization, and Penetration Ability /

The need for novel drug delivery peptides is an important issue of the modern pharmaceutical research. Here, we test K-rich peptides from plant dehydrin ERD14 (ERD-A, ERD-B, and ERD-C) and the C-terminal CPP-resembling region of S100A4 (S100) using the 5(6)-carboxyfluorescein (Cf) tag at the N-termi...

Teljes leírás

Elmentve itt :
Bibliográfiai részletek
Szerzők: Sebák Fanni
Horváth Lilla Borbála
Kovács Dániel
Szolomájer János
Tóth Gábor
Babiczky Ákos
Bősze Szilvia
Bodor Andrea
Dokumentumtípus: Cikk
Megjelent: 2021
Sorozat:ACS OMEGA 6 No. 50
Tárgyszavak:
doi:10.1021/acsomega.1c04637

mtmt:32491001
Online Access:http://publicatio.bibl.u-szeged.hu/24286
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245 1 0 |a Novel Lysine-Rich Delivery Peptides of Plant Origin ERD and Human S100  |h [elektronikus dokumentum] :  |b The Effect of Carboxyfluorescein Conjugation, Influence of Aromatic and Proline Residues, Cellular Internalization, and Penetration Ability /  |c  Sebák Fanni 
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520 3 |a The need for novel drug delivery peptides is an important issue of the modern pharmaceutical research. Here, we test K-rich peptides from plant dehydrin ERD14 (ERD-A, ERD-B, and ERD-C) and the C-terminal CPP-resembling region of S100A4 (S100) using the 5(6)-carboxyfluorescein (Cf) tag at the N-terminus. Via a combined pH-dependent NMR and fluorescence study, we analyze the effect of the Cf conjugation/modification on the structural behavior, separately investigating the (5)-Cf and (6)-Cf forms. Flow cytometry results show that all peptides internalize; however, there is a slight difference between the cellular internalization of (5)- and (6)-Cf-peptides. We indicate the possible importance of residues with an aromatic sidechain and proline. We prove that ERD-A localizes mostly in the cytosol, ERD-B and S100 have partial colocalization with lysosomal staining, and ERD-C mainly localizes within vesicle-like compartments, while the uptake mechanism mainly occurs through energy-dependent paths. 
650 4 |a Kémiai tudományok 
650 4 |a Általános orvostudomány 
700 0 1 |a Horváth Lilla Borbála  |e aut 
700 0 1 |a Kovács Dániel  |e aut 
700 0 1 |a Szolomájer János  |e aut 
700 0 1 |a Tóth Gábor  |e aut 
700 0 1 |a Babiczky Ákos  |e aut 
700 0 1 |a Bősze Szilvia  |e aut 
700 0 1 |a Bodor Andrea  |e aut 
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