A eukaryotic nicotinate-inducible gene cluster convergent evolution in fungi and bacteria /

Nicotinate degradation has hitherto been elucidated only in bacteria. In the ascomycete Aspergillus nidulans, six loci, hxnS/AN9178 encoding the molybdenum cofactor-containing nicotinate hydroxylase, AN11197 encoding a Cys2/His2 zinc finger regulator HxnR, together with AN11196/hxnZ, AN11188/hxnY, A...

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Elmentve itt :
Bibliográfiai részletek
Szerzők: Ámon Judit
Fernandez-Martin Rafael
Bokor Eszter
Cultrone Antonietta
Kelly Joan M.
Flipphi Michel
Scazzocchio Claudio
Kozma-Bognárné Hamari Zsuzsanna
Dokumentumtípus: Cikk
Megjelent: 2017
Sorozat:OPEN BIOLOGY 7 No. 12
doi:10.1098/rsob.170199

mtmt:3323635
Online Access:http://publicatio.bibl.u-szeged.hu/21298
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100 1 |a Ámon Judit 
245 1 2 |a A eukaryotic nicotinate-inducible gene cluster  |h [elektronikus dokumentum] :  |b convergent evolution in fungi and bacteria /  |c  Ámon Judit 
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490 0 |a OPEN BIOLOGY  |v 7 No. 12 
520 3 |a Nicotinate degradation has hitherto been elucidated only in bacteria. In the ascomycete Aspergillus nidulans, six loci, hxnS/AN9178 encoding the molybdenum cofactor-containing nicotinate hydroxylase, AN11197 encoding a Cys2/His2 zinc finger regulator HxnR, together with AN11196/hxnZ, AN11188/hxnY, AN11189/hxnP and AN9177/hxnT, are clustered and stringently co-induced by a nicotinate derivative and subject to nitrogen metabolite repression mediated by the GATA factor AreA. These genes are strictly co-regulated by HxnR. Within the hxnR gene, constitutive mutations map in two discrete regions. Aspergillus nidulans is capable of using nicotinate and its oxidation products 6-hydroxynicotinic acid and 2,5-dihydroxypyridine as sole nitrogen sources in an HxnR-dependent way. HxnS is highly similar to HxA, the canonical xanthine dehydrogenase (XDH), and has originated by gene duplication, preceding the origin of the Pezizomycotina. This cluster is conserved with some variations throughout the Aspergillaceae. Our results imply that a fungal pathway has arisen independently from bacterial ones. Significantly, the neo-functionalization of XDH into nicotinate hydroxylase has occurred independently from analogous events in bacteria. This work describes for the first time a gene cluster involved in nicotinate catabolism in a eukaryote and has relevance for the formation and evolution of co-regulated primary metabolic gene clusters and the microbial degradation of N-heterocyclic compounds. 
700 0 2 |a Fernandez-Martin Rafael  |e aut 
700 0 2 |a Bokor Eszter  |e aut 
700 0 2 |a Cultrone Antonietta  |e aut 
700 0 2 |a Kelly Joan M.  |e aut 
700 0 2 |a Flipphi Michel  |e aut 
700 0 2 |a Scazzocchio Claudio  |e aut 
700 0 2 |a Kozma-Bognárné Hamari Zsuzsanna  |e aut 
856 4 0 |u http://publicatio.bibl.u-szeged.hu/21298/1/2017_Open_Biology_Amon_et_al.pdf  |z Dokumentum-elérés