Purkinje cell number-correlated cerebrocerebellar circuit anomaly in the valproate model of autism
While cerebellar alterations may play a crucial role in the development of core autism spectrum disorder (ASD) symptoms, their pathophysiology on the function of cerebrocerebellar circuit loops is largely unknown. We combined multimodal MRI (9.4 T) brain assessment of the prenatal rat valproate (VPA...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2019
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| Sorozat: | SCIENTIFIC REPORTS
9 No. 1 |
| doi: | 10.1038/s41598-019-45667-1 |
| mtmt: | 30816395 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/19011 |
| LEADER | 02631nab a2200361 i 4500 | ||
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| 024 | 7 | |a 10.1038/s41598-019-45667-1 |2 doi | |
| 024 | 7 | |a 30816395 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
| 041 | |a zxx | ||
| 100 | 1 | |a Spisák Tamás | |
| 245 | 1 | 0 | |a Purkinje cell number-correlated cerebrocerebellar circuit anomaly in the valproate model of autism |h [elektronikus dokumentum] / |c Spisák Tamás |
| 260 | |c 2019 | ||
| 300 | |a Azonosító: 9225-Terjedelem: 15 p | ||
| 490 | 0 | |a SCIENTIFIC REPORTS |v 9 No. 1 | |
| 520 | 3 | |a While cerebellar alterations may play a crucial role in the development of core autism spectrum disorder (ASD) symptoms, their pathophysiology on the function of cerebrocerebellar circuit loops is largely unknown. We combined multimodal MRI (9.4 T) brain assessment of the prenatal rat valproate (VPA) model and correlated immunohistological analysis of the cerebellar Purkinje cell number to address this question. We hypothesized that a suitable functional MRI (fMRI) paradigm might show some altered activity related to disrupted cerebrocerebellar information processing. Two doses of maternal VPA (400 and 600 mg/kg, s.c.) were used. The higher VPA dose induced 3% smaller whole brain volume, the lower dose induced 2% smaller whole brain volume and additionally a focal gray matter density decrease in the cerebellum and brainstem. Increased cortical BOLD responses to whisker stimulation were detected in both VPA groups, but it was more pronounced and extended to cerebellar regions in the 400 mg/kg VPA group. Immunohistological analysis revealed a decreased number of Purkinje cells in both VPA groups. In a detailed analysis, we revealed that the Purkinje cell number interacts with the cerebral BOLD response distinctively in the two VPA groups that highlights atypical function of the cerebrocerebellar circuit loops with potential translational value as an ASD biomarker. | |
| 700 | 0 | 1 | |a Román Viktor |e aut |
| 700 | 0 | 1 | |a Papp Edit |e aut |
| 700 | 0 | 1 | |a Kedves Rita |e aut |
| 700 | 0 | 1 | |a Sághy Katalin |e aut |
| 700 | 0 | 1 | |a Csölle Cecília Katalin |e aut |
| 700 | 0 | 1 | |a Varga Anita |e aut |
| 700 | 0 | 1 | |a Gajári Dávid |e aut |
| 700 | 0 | 1 | |a Nyitrai Gabriella |e aut |
| 700 | 0 | 1 | |a Spisák Zsófa |e aut |
| 700 | 0 | 1 | |a Kincses Zsigmond Tamás |e aut |
| 700 | 0 | 1 | |a Lévay György István |e aut |
| 700 | 0 | 1 | |a Lendvai Balázs |e aut |
| 700 | 0 | 1 | |a Czurkó András |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/19011/1/SpisakT.-Purkinjecellnumber-correlated.....pdf |z Dokumentum-elérés |