State-of-the-art enantioseparations of natural and unnatural amino acids by high-performance liquid chromatography

This review discusses recent publications on the separation and analysis of natural and unnatural amino acid enantiomers by liquid chromatography. Focus is placed on methodological aspects relating chiral stationary phases and chiral columns which can cope with the challenge. Conceptually, amino aci...

Full description

Saved in:
Bibliographic Details
Main Authors: Ilisz István
Péter Antal
Lindner Wolfgang
Format: Article
Published: 2016
Series:TRAC-TRENDS IN ANALYTICAL CHEMISTRY 81
doi:10.1016/j.trac.2016.01.016

mtmt:3103586
Online Access:http://publicatio.bibl.u-szeged.hu/18516
LEADER 01734nab a2200229 i 4500
001 publ18516
005 20200408141058.0
008 200408s2016 hu o 0|| zxx d
022 |a 0165-9936 
024 7 |a 10.1016/j.trac.2016.01.016  |2 doi 
024 7 |a 3103586  |2 mtmt 
040 |a SZTE Publicatio Repozitórium  |b hun 
041 |a zxx 
100 1 |a Ilisz István 
245 1 0 |a State-of-the-art enantioseparations of natural and unnatural amino acids by high-performance liquid chromatography  |h [elektronikus dokumentum] /  |c  Ilisz István 
260 |c 2016 
300 |a 11-22 
490 0 |a TRAC-TRENDS IN ANALYTICAL CHEMISTRY  |v 81 
520 3 |a This review discusses recent publications on the separation and analysis of natural and unnatural amino acid enantiomers by liquid chromatography. Focus is placed on methodological aspects relating chiral stationary phases and chiral columns which can cope with the challenge. Conceptually, amino acids can be enantioseparated in free form, which refers to the resolution of polar ampholytes, or as N-protected amino acids, which can be regarded as acidic commodities. Such synthons are used, for instance, in peptide synthesis protocols. Amino groups are frequently tagged with highly fluorescent or MS/MS active labels in order to generate sensitive and simultaneously stereoselective assays of diverse amino acids in complex matrices. It is our intention to present the state of the art of enantioselective amino acid analysis by HPLC concepts and to pinpoint practical aspects. (C) 2016 Elsevier B.V. All rights reserved. 
700 0 1 |a Péter Antal  |e aut 
700 0 1 |a Lindner Wolfgang  |e aut 
856 4 0 |u http://publicatio.bibl.u-szeged.hu/18516/1/Ilisz_TrAC_81_2016_11_22.pdf  |z Dokumentum-elérés