Molecular characterization of multidrug-resistant Bacteroides isolates from Hungarian clinical samples

INTRODUCTION: Members of the Bacteroides fragilis group are the most important components of the normal human gut microbiota, but these bacteria can cause severe infections as well. Due to the frequent usage of antibiotics, the spreading of the MDR strains is a real threat worldwide. MATERIALS AND M...

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Main Authors: Sárvári Károly Péter
Sóki József
Kristóf Katalin
Juhász Emese
Miszti Cecilia
Melegh Szilvia Zsóka
Latkoczy Krisztina
Zsoldiné Urbán Edit
Format: Article
Published: 2018
Series:JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE 13
doi:10.1016/j.jgar.2017.10.020

mtmt:3287393
Online Access:http://publicatio.bibl.u-szeged.hu/17690
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520 3 |a INTRODUCTION: Members of the Bacteroides fragilis group are the most important components of the normal human gut microbiota, but these bacteria can cause severe infections as well. Due to the frequent usage of antibiotics, the spreading of the MDR strains is a real threat worldwide. MATERIALS AND METHODS: In a multicentre study 400 Bacteroides isolates from five Hungarian microbiological laboratories were cultured and identified by MALDI TOF-MS. The MIC values of ten antibiotics were determined by the agar dilution method and evaluated by the breakpoints of EUCAST or CLSI. RESULTS: We found six MDR strains, and their antibiotic resistance genes were investigated by molecular methods The DNA amplicon of B. fragilis SZ38 strain was sequenced to look for a mutation in the gyrA gene. Among the six MDR isolates we found one cfiA-, two cepA, three cfxA-, two ermG-, six tetQ- three tetX- and two bexA-positive strains. None of them harboured cepA, nim, ermB and tetX1 genes. DISCUSSION: In the past 12 years only a few cases of MDR Bacteroides infections have been published. Within a comprehensive multicenter survey we demonstrated the relatively large prevalence of MDR strains isolated in one centre with five isolates and one from another centre during a relatively short period of time. This study focused on the importance of antimicrobial susceptibility testing and surveillance among B. fragilis group isolates. 
700 0 1 |a Sóki József  |e aut 
700 0 1 |a Kristóf Katalin  |e aut 
700 0 1 |a Juhász Emese  |e aut 
700 0 1 |a Miszti Cecilia  |e aut 
700 0 1 |a Melegh Szilvia Zsóka  |e aut 
700 0 1 |a Latkoczy Krisztina  |e aut 
700 0 2 |a Zsoldiné Urbán Edit  |e aut 
856 4 0 |u http://publicatio.bibl.u-szeged.hu/17690/1/10.1016j.jgar.2017.10.020.pdf  |z Dokumentum-elérés