Upregulation of K(2P)3.1 K+ Current Causes Action Potential Shortening in Patients With Chronic Atrial Fibrillation
Background-Antiarrhythmic management of atrial fibrillation (AF) remains a major clinical challenge. Mechanism-based approaches to AF therapy are sought to increase effectiveness and to provide individualized patient care. K(2P)3.1 (TASK-1 [tandem of P domains in a weak inward-rectifying K+ channel-...
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| Format: | Article |
| Published: |
2015
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| Series: | CIRCULATION
132 No. 2 |
| doi: | 10.1161/CIRCULATIONAHA.114.012657 |
| mtmt: | 3181027 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/16229 |
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| 024 | 7 | |a 3181027 |2 mtmt | |
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| 100 | 1 | |a Schmidt Constanze | |
| 245 | 1 | 0 | |a Upregulation of K(2P)3.1 K+ Current Causes Action Potential Shortening in Patients With Chronic Atrial Fibrillation |h [elektronikus dokumentum] / |c Schmidt Constanze |
| 260 | |c 2015 | ||
| 300 | |a 82-92 | ||
| 490 | 0 | |a CIRCULATION |v 132 No. 2 | |
| 520 | 3 | |a Background-Antiarrhythmic management of atrial fibrillation (AF) remains a major clinical challenge. Mechanism-based approaches to AF therapy are sought to increase effectiveness and to provide individualized patient care. K(2P)3.1 (TASK-1 [tandem of P domains in a weak inward-rectifying K+ channel-related acid-sensitive K+ channel-1]) 2-pore-domain K+ (K-2P) channels have been implicated in action potential regulation in animal models. However, their role in the pathophysiology and treatment of paroxysmal and chronic patients with AF is unknown. Methods and Results-Right and left atrial tissue was obtained from patients with paroxysmal or chronic AF and from control subjects in sinus rhythm. Ion channel expression was analyzed by quantitative real-time polymerase chain reaction and Western blot. Membrane currents and action potentials were recorded using voltage-and current-clamp techniques. K(2P)3.1 subunits exhibited predominantly atrial expression, and atrial K(2P)3.1 transcript levels were highest among functional K-2P channels. K(2P)3.1 mRNA and protein levels were increased in chronic AF. Enhancement of corresponding currents in the right atrium resulted in shortened action potential duration at 90% of repolarization (APD(90)) compared with patients in sinus rhythm. In contrast, K(2P)3.1 expression was not significantly affected in subjects with paroxysmal AF. Pharmacological K(2P)3.1 inhibition prolonged APD(90) in atrial myocytes from patients with chronic AF to values observed among control subjects in sinus rhythm. Conclusions-Enhancement of atrium-selective K(2P)3.1 currents contributes to APD shortening in patients with chronic AF, and K(2P)3.1 channel inhibition reverses AF-related APD shortening. These results highlight the potential of K(2P)3.1 as a novel drug target for mechanism-based AF therapy. | |
| 700 | 0 | 1 | |a Wiedmann Felix |e aut |
| 700 | 0 | 1 | |a Voigt Niels |e aut |
| 700 | 0 | 1 | |a Zhou Xiao-Bo |e aut |
| 700 | 0 | 1 | |a Heijman Jordi |e aut |
| 700 | 0 | 1 | |a Lang Siegfried |e aut |
| 700 | 0 | 1 | |a Albert Virginia |e aut |
| 700 | 0 | 1 | |a Kallenberger Stefan |e aut |
| 700 | 0 | 1 | |a Ruhparwar Arjang |e aut |
| 700 | 0 | 1 | |a Szabó Gábor Balázs |e aut |
| 700 | 0 | 1 | |a Kallenbach Klaus |e aut |
| 700 | 0 | 1 | |a Karck Matthias |e aut |
| 700 | 0 | 1 | |a Borggrefe Martin |e aut |
| 700 | 0 | 1 | |a Biliczki Péter |e aut |
| 700 | 0 | 1 | |a Baczkó István |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/16229/1/SchmidtCirculation2015.pdf |z Dokumentum-elérés |