Rutin and Its Cyclodextrin Inclusion Complexes Physico-chemical Evaluation and in vitro Activity on B164A5 Murine Melanoma Cell Line. /
BACKGROUND: Recent studies have shown that rutin presents a heightened interest due to the plethora of biological activities. The major drawback for this phytocompound is the poor water solubility, a parameter that limits its bioavailability. The study aimed to prepare and assess the inclusion compl...
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| Format: | Article |
| Published: |
2017
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| Series: | CURRENT PHARMACEUTICAL BIOTECHNOLOGY
18 No. 13 |
| doi: | 10.2174/1389201019666180209165523 |
| mtmt: | 3375794 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/14777 |
| LEADER | 02433nab a2200313 i 4500 | ||
|---|---|---|---|
| 001 | publ14777 | ||
| 005 | 20190305144943.0 | ||
| 008 | 190305s2017 hu o 0|| angol d | ||
| 022 | |a 1389-2010 | ||
| 024 | 7 | |a 10.2174/1389201019666180209165523 |2 doi | |
| 024 | 7 | |a 3375794 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
| 041 | |a angol | ||
| 100 | 1 | |a Corina Danciu | |
| 245 | 1 | 0 | |a Rutin and Its Cyclodextrin Inclusion Complexes |h [elektronikus dokumentum] : |b Physico-chemical Evaluation and in vitro Activity on B164A5 Murine Melanoma Cell Line. / |c Corina Danciu |
| 260 | |c 2017 | ||
| 300 | |a 1067-1077 | ||
| 490 | 0 | |a CURRENT PHARMACEUTICAL BIOTECHNOLOGY |v 18 No. 13 | |
| 520 | 3 | |a BACKGROUND: Recent studies have shown that rutin presents a heightened interest due to the plethora of biological activities. The major drawback for this phytocompound is the poor water solubility, a parameter that limits its bioavailability. The study aimed to prepare and assess the inclusion complexes of rutin with beta-cyclodextrin (BCD) and hydroxypropyl-beta-cyclodextrin (HPBCD), followed by the evaluation of the antioxidant, antiproliferative and pro-apoptotic activity against B164A5 murine melanoma cell line. METHODS: Inclusion complexes were prepared by kneading method. Scanning electron microscopy, differential scanning calorimetry and X-ray powder diffraction were used in order to assess their formation. Antioxidant activity was determined by DPPH assay. Antiproliferative and pro-apoptotic effects where tested by MTT, respectively Annexin V-PI assays. Physico-chemical methods proved that incorporation took place. RESULTS: Results show that rutin presents antioxidant activity, and complexation increases this property. After 72 h of incubation at the concentration of 100 microM rutin proved to be an active antiproliferative and pro-apoptotic compound against B164A5 cells. CONCLUSION: Incorporation in BCD and HBCD maintained the activity, thus representing an important finding and open path for in vivo experiments. | |
| 700 | 0 | 1 | |a Florina Bojin |e aut |
| 700 | 0 | 1 | |a Iulia Pinzaru |e aut |
| 700 | 0 | 1 | |a Cristina Dehelean |e aut |
| 700 | 0 | 1 | |a Ambrus Rita |e aut |
| 700 | 0 | 1 | |a Alexandra Popescu |e aut |
| 700 | 0 | 1 | |a Virgil Paunescu |e aut |
| 700 | 0 | 1 | |a Hancianu Monica |e aut |
| 700 | 0 | 1 | |a Daliana Monica |e aut |
| 700 | 0 | 1 | |a Codruta Soica |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/14777/1/Corina.pdf |z Dokumentum-elérés |