Structural optimization of foldamer-dendrimer conjugates as multivalent agents against the toxic effects of amyloid beta oligomers

Alzheimer's disease is one of the most common chronic neurodegenerative disorders. Despite several in vivo and clinical studies, the cause of the disease is poorly understood. Currently, amyloid β (Aβ) peptide and its tendency to assemble into soluble oligomers are known as a main pathogenic ev...

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Bibliographic Details
Main Authors: Bartus Éva
Olajos Gábor
Schuster Ildikó
Bozsó Zsolt
Deli Mária Anna
Veszelka Szilvia
Walter Fruzsina
Datki Zsolt László
Szakonyi Zsolt
Martinek Tamás
Fülöp Lívia
Format: Article
Published: 2018
Series:MOLECULES 23 No. 10
doi:10.3390/molecules23102523

mtmt:30310687
Online Access:http://publicatio.bibl.u-szeged.hu/14057
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520 3 |a Alzheimer's disease is one of the most common chronic neurodegenerative disorders. Despite several in vivo and clinical studies, the cause of the disease is poorly understood. Currently, amyloid β (Aβ) peptide and its tendency to assemble into soluble oligomers are known as a main pathogenic event leading to the interruption of synapses and brain degeneration. Targeting neurotoxic Aβ oligomers can help recognize the disease at an early stage or it can be a potential therapeutic approach. Unnatural β-peptidic foldamers are successfully used against many different protein targets due to their favorable structural and pharmacokinetic properties compared to small molecule or protein-like drug candidates. We have previously reported a tetravalent foldamer-dendrimer conjugate which can selectively bind Aβ oligomers. Taking advantage of multivalency and foldamers, we synthesized different multivalent foldamer-based conjugates to optimize the geometry of the ligand. Isothermal titration calorimetry (ITC) was used to measure binding affinity to Aβ, thereafter 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) based tissue viability assay and impedance-based viability assay on SH-SY5Y cells were applied to monitor Aβ toxicity and protective effects of the compounds. Important factors for high binding affinity were determined and a good correlation was found between influencing the valence and the capability of the conjugates for Aβ binding. 
700 0 1 |a Olajos Gábor  |e aut 
700 0 1 |a Schuster Ildikó  |e aut 
700 0 1 |a Bozsó Zsolt  |e aut 
700 0 1 |a Deli Mária Anna  |e aut 
700 0 1 |a Veszelka Szilvia  |e aut 
700 0 1 |a Walter Fruzsina  |e aut 
700 0 1 |a Datki Zsolt László  |e aut 
700 0 1 |a Szakonyi Zsolt  |e aut 
700 0 1 |a Martinek Tamás  |e aut 
700 0 1 |a Fülöp Lívia  |e aut 
856 4 0 |u http://publicatio.bibl.u-szeged.hu/14057/1/molecules-23-02523.pdf  |z Dokumentum-elérés