Mátrix-asszisztált lézer deszorpciós, ionizaciós, repülési idő mérésen alapuló tömegspektrometria speciális alkalmazása a klinikai mikrobiológiai diagnosztika területen

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry as a new possibility for rapid identification of bacteria and fungi revolutionized the clinical microbiological diagnostics. It has an extreme importance in the routine microbiological laboratories, as identification of the...

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Bibliographic Details
Main Authors: Nagy Erzsébet
Pappné Ábrók Marianna
Bartha Noémi Anikó
Bereczki László
Juhász Emese
Kardos Gábor
Kristóf Katalin
Miszti Cecília
Zsoldiné Urbán Edit
Format: Article
Published: Akadémiai Kiadó 2014
Series:ORVOSI HETILAP 155 No. 38
doi:10.1556/OH.2014.29985

mtmt:2736245
Online Access:http://publicatio.bibl.u-szeged.hu/12184
Description
Summary:Matrix-assisted laser desorption ionization time-of-flight mass spectrometry as a new possibility for rapid identification of bacteria and fungi revolutionized the clinical microbiological diagnostics. It has an extreme importance in the routine microbiological laboratories, as identification of the pathogenic species rapidly will influence antibiotic selection before the final determination of antibiotic resistance of the isolate. The classical methods for identification of bacteria or fungi, based on biochemical tests, are influenced by many environmental factors. The matrix-assisted laser desorption ionization time-of-flight mass spectrometry is a rapid method which is able to identify a great variety of the isolated bacteria and fungi based on the composition of conserved ribosomal proteins. Recently several other applications of the method have also been investigated such as direct identification of pathogens from the positive blood cultures. There are possibilities to identify bacteria from the urine samples in urinary tract infection or from other sterile body fluids. Using selective enrichment broth Salmonella sp from the stool samples can be identified more rapidly, too. The extended spectrum beta-lactamase or carbapenemase production of the isolated bacteria can be also detected by this method helping the antibiotic selection in some cases. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry based methods are suitable to investigate changes in deoxyribonucleic acid or ribonucleic acid, to carry out rapid antibiotic resistance determination or other proteomic analysis. The aim of this paper is to give an overview about present possibilities of using this technique in the clinical microbiological routine procedures. Orv. Hetil., 2014, 155(38), 1495-1503.
Physical Description:1495-1503
ISSN:0030-6002