Expression of SERCA2a is independent of innervation in regenerating soleus muscle
The speed of contraction of a skeletal muscle largely depends on the myosin heavy chain isoforms (MyHC), whereas the relaxation is initiated and maintained by the sarcoplasmic reticulum Ca2+-ATPases (SERCA). The expression of the slow muscle-type myosin heavy chain I (MyHCI) is entirely dependent on...
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2003
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| Sorozat: | AMERICAN JOURNAL OF PHYSIOLOGY: CELL PHYSIOLOGY
285 No. 4 |
| doi: | 10.1152/ajpcell.00592.2002 |
| mtmt: | 1956124 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/10891 |
| LEADER | 02481nab a2200217 i 4500 | ||
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| 001 | publ10891 | ||
| 005 | 20190716083931.0 | ||
| 008 | 170302s2003 hu o 0|| angol d | ||
| 022 | |a 0363-6143 | ||
| 024 | 7 | |a 10.1152/ajpcell.00592.2002 |2 doi | |
| 024 | 7 | |a 1956124 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
| 041 | |a angol | ||
| 100 | 1 | |a Zádor Ernő | |
| 245 | 1 | 0 | |a Expression of SERCA2a is independent of innervation in regenerating soleus muscle |h [elektronikus dokumentum] / |c Zádor Ernő |
| 260 | |c 2003 | ||
| 300 | |a C853-C861 | ||
| 490 | 0 | |a AMERICAN JOURNAL OF PHYSIOLOGY: CELL PHYSIOLOGY |v 285 No. 4 | |
| 520 | 3 | |a The speed of contraction of a skeletal muscle largely depends on the myosin heavy chain isoforms (MyHC), whereas the relaxation is initiated and maintained by the sarcoplasmic reticulum Ca2+-ATPases (SERCA). The expression of the slow muscle-type myosin heavy chain I (MyHCI) is entirely dependent on innervation, but, as we show here, innervation is not required for the expression of the slow-type sarcoplasmic reticulum Ca2+-ATPase (SERCA2a) in regenerating soleus muscles of the rat, although it can play a modulator role. Remarkably, the SERCA2a level is even higher in denervated than in innervated regenerating soleus muscles on day 7 when innervation is expected to resume. Later, the level of SERCA2a protein declines in denervated regenerated muscles but it remains expressed, whereas the corresponding mRNA level is still increasing. SERCA1 (i.e., the fast muscle-type isoform) expression shows only minor changes in denervated regenerating soleus muscles compared with innervated regenerating controls. When the soleus nerve was transected instead of the sciatic nerve, SERCA2a and MyHCI expressions were found to be even more uncoupled because the MyHCI nearly completely disappeared, whereas the SERCA2a mRNA and protein levels decreased much less. The transfection of regenerating muscles with constitutively active mutants of the Ras oncogene, known to mimic the effect of innervation on the expression of MyHCI, did not affect SERCA2a expression. These results demonstrate that the regulation of SERCA2a expression is clearly distinct from that of the slow myosin in the regenerating soleus muscle and that SERCA2a expression is modulated by neuronal activity but is not entirely dependent on it. | |
| 700 | 0 | 1 | |a Wuytack Frank |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/10891/1/2003_Zador_and_Wuytack_AJP_u.pdf |z Dokumentum-elérés |