Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Signalling Enhances Osteogenesis in UMR-106 Cell Line

Presence of the pituitary adenylate cyclase-activating polypeptide (PACAP) signalling has been proved in various peripheral tissues. PACAP can activate protein kinase A (PKA) signalling via binding to pituitary adenylate cyclase-activating polypeptide type I receptor (PAC1), vasoactive intestinal po...

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Main Authors: Juhász Tamás
Matta Csaba
Mészár-Katona Éva
Somogyi Csilla
Takács Roland Ádám
Hajdú Tibor
Helgadottir Solveig Lind
Fodor János
Csernoch László
Tóth Gábor
Bakó Éva
Reglődi Dóra
Tamás Andrea
Zákány Róza
Format: Article
Published: 2014
Series:JOURNAL OF MOLECULAR NEUROSCIENCE 54 No. 3
doi:10.1007/s12031-014-0389-1

mtmt:2719815
Online Access:http://publicatio.bibl.u-szeged.hu/10467
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520 3 |a Presence of the pituitary adenylate cyclase-activating polypeptide (PACAP) signalling has been proved in various peripheral tissues. PACAP can activate protein kinase A (PKA) signalling via binding to pituitary adenylate cyclase-activating polypeptide type I receptor (PAC1), vasoactive intestinal polypeptide receptor (VPAC) 1 or VPAC2 receptor. Since little is known about the role of this regulatory mechanism in bone formation, we aimed to investigate the effect of PACAP on osteogenesis of UMR-106 cells. PACAP 1-38 as an agonist and PACAP 6-38 as an antagonist of PAC1 were added to the culture medium. Surprisingly, both substances enhanced protein expressions of collagen type I, osterix and alkaline phosphatase, along with higher cell proliferation rate and an augmented mineralisation. Although expression of PKA was elevated, no alterations were detected in the expression, phosphorylation and nuclear presence of CREB, but increased nuclear appearance of Runx2, the key transcription factor of osteoblast differentiation, was shown. Both PACAPs increased the expressions of bone morphogenetic proteins (BMPs) 2, 4, 6, 7 and Smad1 proteins, as well as that of Sonic hedgehog, PATCH1 and Gli1. Data of our experiments indicate that activation of PACAP pathway enhances bone formation of UMR-106 cells and PKA, BMP and Hedgehog signalling pathways became activated. We also found that PACAP 6-38 did not act as an antagonist of PACAP signalling in UMR-106 cells. 
700 0 1 |a Matta Csaba  |e aut 
700 0 2 |a Mészár-Katona Éva  |e aut 
700 0 2 |a Somogyi Csilla  |e aut 
700 0 2 |a Takács Roland Ádám  |e aut 
700 0 2 |a Hajdú Tibor  |e aut 
700 0 2 |a Helgadottir Solveig Lind  |e aut 
700 0 2 |a Fodor János  |e aut 
700 0 2 |a Csernoch László  |e aut 
700 0 2 |a Tóth Gábor  |e aut 
700 0 2 |a Bakó Éva  |e aut 
700 0 2 |a Reglődi Dóra  |e aut 
700 0 2 |a Tamás Andrea  |e aut 
700 0 2 |a Zákány Róza  |e aut 
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