Investigations on normal human adult epidermal melanoytes
For obtaining pure melanocyte cultures from human adult epidermis a novel culture technique using a cholera toxin and PMA-free medium (Mel-mix) was described by our group. In Mel-mix medium the cultured melanocytes become bipolar, unpigmented and highly proliferative. Further characterization of the...
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| Dokumentumtípus: | Disszertáció |
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2011-05-04
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| Tárgyszavak: | |
| doi: | 10.14232/phd.745 |
| mtmt: | 1922994 |
| Online Access: | http://doktori.ek.szte.hu/745 |
| LEADER | 02237nta a2200229 i 4500 | ||
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| 001 | dokt745 | ||
| 005 | 20190716145605.0 | ||
| 008 | 110419s2011 hu om 0|| eng d | ||
| 024 | 7 | |a 10.14232/phd.745 |2 doi | |
| 024 | 7 | |a 1922994 |2 mtmt | |
| 040 | |a SZTE Doktori Repozitórium |b hun | ||
| 041 | |a eng | ||
| 100 | 1 | |a Kormos Bernadett | |
| 245 | 1 | 0 | |a Investigations on normal human adult epidermal melanoytes |h [elektronikus dokumentum] / |c Kormos Bernadett |
| 246 | 1 | 0 | |a Vizsgálatok egészséges felnőtt epidermális melanocitákon |h [elektronikus dokumentum] |
| 260 | |c 2011-05-04 | ||
| 502 | |a Disszertacio | ||
| 520 | 3 | |a For obtaining pure melanocyte cultures from human adult epidermis a novel culture technique using a cholera toxin and PMA-free medium (Mel-mix) was described by our group. In Mel-mix medium the cultured melanocytes become bipolar, unpigmented and highly proliferative. Further characterization of the cultured melanocytes revealed the disappearance of c-Kit and TRP-1 and induction of nestin expression, indicating that melanocytes dedifferentiated in this in vitro culture. Cholera toxin and PMA were able to induce c-Kit and TRP-1 protein expressions in the cells, reversing dedifferentiation. TRP-1 mRNA expression was induced in dedifferentiated melanocytes by UV-B irradiated keratinocyte supernatants, however direct UV-B irradiation of the cells resulted in further decrease of TRP-1 mRNA expression. Expressions of EGFR mRNA and protein and mitogenic effect of EGF on Mel-mix cultured melanocytes were proved. EGFR protein expression was more intensive in dedifferentiated melanocytes. These dedifferentiated, easily accessible cultured melanocytes provide a good model for studying melanocyte differentiation and possibly transdifferentiation. Because melanocytes in Mel-mix medium can be cultured with human serum as the only supplement, this culture system is also suitable for autologous cell transplantation. | |
| 650 | 4 | |a klinikai orvostudományok | |
| 700 | 2 | |a Bata-Csörgő Zsuzsanna |e ths | |
| 856 | 4 | 0 | |u https://doktori.bibl.u-szeged.hu/id/eprint/745/1/disszert%C3%A1ci%C3%B3_Kormos.pdf |z Dokumentum-elérés |
| 856 | 4 | 0 | |u https://doktori.bibl.u-szeged.hu/id/eprint/745/2/t%C3%A9zis_Kormos.pdf |z Dokumentum-elérés |