A newly identified specific biological activity of glyphosate - inhibition of RGD-binding integrins
In this study we investigated the inhibitory effect of the widely used broad-spectrum herbicide active ingredient glyphosate and its related analogues on αVβ3 integrin binding to the shortest oligopeptide recognizing motif of integrins, the arginine-glycine-aspartic acid (RGD) sequence. Integrin bin...
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| Dokumentumtípus: | Könyv része |
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2022
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| Sorozat: | Proceedings of the International Symposium on Analytical and Environmental Problems
28 |
| Kulcsszavak: | Kémia |
| Tárgyszavak: | |
| Online Access: | http://acta.bibl.u-szeged.hu/78491 |
| LEADER | 02451naa a2200301 i 4500 | ||
|---|---|---|---|
| 001 | acta78491 | ||
| 005 | 20230320130404.0 | ||
| 008 | 230320s2022 hu o 1|| eng d | ||
| 020 | |a 978-963-306-904-2 | ||
| 040 | |a SZTE Egyetemi Kiadványok Repozitórium |b hun | ||
| 041 | |a eng | ||
| 100 | 1 | |a Gémes Borbála | |
| 245 | 1 | 2 | |a A newly identified specific biological activity of glyphosate - inhibition of RGD-binding integrins |h [elektronikus dokumentum] / |c Gémes Borbála |
| 260 | |c 2022 | ||
| 300 | |a 147-151 | ||
| 490 | 0 | |a Proceedings of the International Symposium on Analytical and Environmental Problems |v 28 | |
| 520 | 3 | |a In this study we investigated the inhibitory effect of the widely used broad-spectrum herbicide active ingredient glyphosate and its related analogues on αVβ3 integrin binding to the shortest oligopeptide recognizing motif of integrins, the arginine-glycine-aspartic acid (RGD) sequence. Integrin binding characteristics were assessed in a modified enzyme linked immunosorbent assay (ELISA) and by a label-free optical biosensor technique. At 22 mM, glyphosate reached full inhibition of αVβ3, and the inhibitory activity of its main metabolite, aminomethylphosphonic acid (AMPA) was also above 95%, while another environmentally relevant metabolite, sarcosine exerted only a weaker effect, approximately 35% inhibition. In turn, the half maximal inhibitory concentration (IC50) of glyphosate and AMPA were reported to be 2.7±0.5 mM and 1.3±0.2 mM, respectively. The inhibitory effects of the other related compounds investigated (acetylglycine, glycine and iminodiacetic acid) at the same concentration, 22 mM were below 50%. Inhibitory effects on cell adhesion to RGD-modified surfaces by whole cells containing several types of RGD-binding integrins including αVβ3 were detected using the biosensor technique, where the integrin antagonist activity of glyphosate was also demonstrated. | |
| 650 | 4 | |a Természettudományok | |
| 650 | 4 | |a Kémiai tudományok | |
| 695 | |a Kémia | ||
| 700 | 0 | 1 | |a Takács Eszter |e aut |
| 700 | 0 | 1 | |a Székács Inna |e aut |
| 700 | 0 | 1 | |a Farkas Enikő |e aut |
| 700 | 0 | 1 | |a Horváth Róbert |e aut |
| 700 | 0 | 1 | |a Klátyik Szandra |e aut |
| 700 | 0 | 1 | |a Székács András |e aut |
| 711 | |a 28th International Symposium on Analytical and Environmental Problems |c Szeged |d 2022. november 14-15. | ||
| 856 | 4 | 0 | |u http://acta.bibl.u-szeged.hu/78491/1/proceedings_of_isaep_2022_147-151.pdf |z Dokumentum-elérés |