Waste-wood derived biochar as a support for horseradish peroxidase immobilization

In this paper, the suitability of waste-wood derived biochar particles as a support for the horseradish peroxidase (HRP) immobilization by adsorption method was investigated. The change in enzymatic activity of the immobilized enzyme at different values of pH and temperature, as well as stability ov...

Teljes leírás

Elmentve itt :
Bibliográfiai részletek
Szerzők: Petronijević Mirjana
Panić Sanja
Đurović-Mladenović Nataša
Savić Saša
Testületi szerző: International Symposium on Analytical and Environmental Problems (26.) (2020) (Szeged)
Dokumentumtípus: Könyv része
Megjelent: 2020
Sorozat:Proceedings of the International Symposium on Analytical and Environmental Problems 26
Kulcsszavak:Analitikai kémia
Tárgyszavak:
Online Access:http://acta.bibl.u-szeged.hu/74016
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520 3 |a In this paper, the suitability of waste-wood derived biochar particles as a support for the horseradish peroxidase (HRP) immobilization by adsorption method was investigated. The change in enzymatic activity of the immobilized enzyme at different values of pH and temperature, as well as stability over time, was measured. The results showed that HRP can efficiently bind to biochar particles by adsorption. The immobilized enzyme shows high activity (>80%) at a wide range of pH (7-9) and temperature (20-50°C). The immobilized enzyme retains 22% and 40% of its activity during storage at temperatures of 25 and 10°C after a period of 30 days, respectively. 
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650 4 |a Kémiai tudományok 
695 |a Analitikai kémia 
700 0 1 |a Panić Sanja  |e aut 
700 0 2 |a Đurović-Mladenović Nataša  |e aut 
700 0 2 |a Savić Saša  |e aut 
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