02067nab a2200277 i 4500 acta35913 20260514112756.0 161017s2015 hu o 000 eng d 1588-385X SZTE Egyetemi Kiadványok Repozitórium hun eng Mandal Asish Parametric optimalization of submerged fermantation conditions for xylanase production Bacillus cereus BSA1 through Taguchi Methodology [elektronikus dokumentum] / Mandal Asish University of Szeged Szeged 2015 189-195 Acta biologica Szegediensis 59 No. 2 Extracellular xylanase production of Bacillus cereus BSA1 was optimized under submerged fermentation using Taguchi orthogonal array (OA). An L18 layout of OA was constructed at three-levels of six factors, i.e. temperature, pH, and xylan, Na2HPO4, NH4NO3 and NaCl concentrations, influencing the xylanase synthesis. The enzyme production was studied in 18 parallel batch systems using different levels of each factor. The results were processed with Qualitek-4 software using ‘bigger is better’ quality character, and combination of 35 oC; pH 6.0; and xylan 0.5; NH4NO3 0.5, Na2HPO4, 0.1; NaCl 0.05 concentrations (in w/v %) with a predictive xylanase production of 7.404 U/ml was obtained. Fermentation experiment was performed for further validating the statistical output, and it resulted 10.24% in the xylanase yield (from 6.44 U/ml to 7.10 U/ml) as compared to one variable at a time (OVAT) design. Interaction effects of the factors individually and in combination can be evaluated by using Taguchi method design of experiment. Természettudományok Biológiai tudományok Mikrobiológia - baktériumok, Bacillus cereus Kar Sanjay aut Dutta Tapan aut Pati Bikas Ranjan aut Mondal Keshab Chandra aut Mohapatra Pradeep Kumar Das aut http://acta.bibl.u-szeged.hu/35913/1/59189.pdf Dokumentum-elérés