Parametric optimalization of submerged fermantation conditions for xylanase production Bacillus cereus BSA1 through Taguchi Methodology

Extracellular xylanase production of Bacillus cereus BSA1 was optimized under submerged fermentation using Taguchi orthogonal array (OA). An L18 layout of OA was constructed at three-levels of six factors, i.e. temperature, pH, and xylan, Na2HPO4, NH4NO3 and NaCl concentrations, influencing the xyl...

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Elmentve itt :
Bibliográfiai részletek
Szerzők: Mandal Asish
Kar Sanjay
Dutta Tapan
Pati Bikas Ranjan
Mondal Keshab Chandra
Mohapatra Pradeep Kumar Das
Dokumentumtípus: Cikk
Megjelent: University of Szeged Szeged 2015
Sorozat:Acta biologica Szegediensis 59 No. 2
Kulcsszavak:Mikrobiológia - baktériumok, Bacillus cereus
Tárgyszavak:
Online Access:http://acta.bibl.u-szeged.hu/35913
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245 1 0 |a Parametric optimalization of submerged fermantation conditions for xylanase production Bacillus cereus BSA1 through Taguchi Methodology  |h [elektronikus dokumentum] /  |c  Mandal Asish 
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490 0 |a Acta biologica Szegediensis  |v 59 No. 2 
520 3 |a Extracellular xylanase production of Bacillus cereus BSA1 was optimized under submerged fermentation using Taguchi orthogonal array (OA). An L18 layout of OA was constructed at three-levels of six factors, i.e. temperature, pH, and xylan, Na2HPO4, NH4NO3 and NaCl concentrations, influencing the xylanase synthesis. The enzyme production was studied in 18 parallel batch systems using different levels of each factor. The results were processed with Qualitek-4 software using ‘bigger is better’ quality character, and combination of 35 oC; pH 6.0; and xylan 0.5; NH4NO3 0.5, Na2HPO4, 0.1; NaCl 0.05 concentrations (in w/v %) with a predictive xylanase production of 7.404 U/ml was obtained. Fermentation experiment was performed for further validating the statistical output, and it resulted 10.24% in the xylanase yield (from 6.44 U/ml to 7.10 U/ml) as compared to one variable at a time (OVAT) design. Interaction effects of the factors individually and in combination can be evaluated by using Taguchi method design of experiment. 
650 4 |a Természettudományok 
650 4 |a Biológiai tudományok 
695 |a Mikrobiológia - baktériumok, Bacillus cereus 
700 0 1 |a Kar Sanjay  |e aut 
700 0 1 |a Dutta Tapan  |e aut 
700 0 1 |a Pati Bikas Ranjan  |e aut 
700 0 1 |a Mondal Keshab Chandra  |e aut 
700 0 1 |a Mohapatra Pradeep Kumar Das  |e aut 
856 4 0 |u http://acta.bibl.u-szeged.hu/35913/1/59189.pdf  |z Dokumentum-elérés